Nao Yoshida1, Sueo Niimura2. 1. Graduate School of Science and Technology Niigata University 950-2181 Niigata Japan. 2. Faculty of Agriculture Niigata University 950-2181 Niigata Japan.
Abstract
Purpose: The size of the perivitelline space and the incidence of polyspermy were observed in ovulated and cultured oocytes from rabbits and hamsters with or without treatment by 4-methylumbelliferone (MU), an inhibitor of hyaluronic acid (HA) synthase, in order to examine the relationship between the incidence of polyspermy and the size of the perivitelline space. The amount of HA in the medium with MU-treated hamster oocytes was measured and compared with that in the medium with untreated oocytes. Methods: The perivitelline space of oocytes with 1st polar bodies was measured by use of a micrometer under a microscope, and the incidence of polyspermy was observed in the oocytes after insemination. The amount of HA in the medium was examined using an enzyme-linked immunosorbent assay. The distribution of cortical granules was observed by staining with FITC-conjugated LCA. Results: In both rabbit and hamster, the mean size of the perivitelline space was significantly smaller and the incidence of polyspermy was significantly higher in the cultured and MU-treated oocytes than in the ovulated and untreated oocytes. The mean amount of HA in the medium with MU-treated oocytes (8.96 pg) was significantly smaller than that in the medium with untreated oocytes (21.77 pg). The distribution of cortical granules did not differ among the ovulated, cultured, and MU-treated oocytes. Conclusions: These findings suggest that the size of the perivitelline space is closely related to the incidence of polyspermy, and that the oocyte itself synthesizes and secretes the HA involved in the enlargement of the perivitelline space.
Purpose: The size of the perivitelline space and the incidence of polyspermy were observed in ovulated and cultured oocytes from rabbits and hamsters with or without treatment by 4-methylumbelliferone (MU), an inhibitor of hyaluronic acid (HA) synthase, in order to examine the relationship between the incidence of polyspermy and the size of the perivitelline space. The amount of HA in the medium with MU-treated hamster oocytes was measured and compared with that in the medium with untreated oocytes. Methods: The perivitelline space of oocytes with 1st polar bodies was measured by use of a micrometer under a microscope, and the incidence of polyspermy was observed in the oocytes after insemination. The amount of HA in the medium was examined using an enzyme-linked immunosorbent assay. The distribution of cortical granules was observed by staining with FITC-conjugated LCA. Results: In both rabbit and hamster, the mean size of the perivitelline space was significantly smaller and the incidence of polyspermy was significantly higher in the cultured and MU-treated oocytes than in the ovulated and untreated oocytes. The mean amount of HA in the medium with MU-treated oocytes (8.96 pg) was significantly smaller than that in the medium with untreated oocytes (21.77 pg). The distribution of cortical granules did not differ among the ovulated, cultured, and MU-treated oocytes. Conclusions: These findings suggest that the size of the perivitelline space is closely related to the incidence of polyspermy, and that the oocyte itself synthesizes and secretes the HA involved in the enlargement of the perivitelline space.
Authors: Francesca E Duncan; Karen Schindler; Richard M Schultz; Cecilia S Blengini; Paula Stein; Stephen A Stricker; Gary M Wessel; Carmen J Williams Journal: Mol Hum Reprod Date: 2020-11-01 Impact factor: 4.518