Literature DB >> 29644255

Conditional Knockdown of Proteins Using Auxin-inducible Degron (AID) Fusions in Toxoplasma gondii.

Kevin M Brown1, Shaojun Long1, L David Sibley1.   

Abstract

Toxoplasma gondii is a member of the deadly phylum of protozoan parasites called Apicomplexa. As a model apicomplexan, there is a great wealth of information regarding T. gondii's 8,000+ protein coding genes including sequence variation, expression, and relative contribution to parasite fitness. However, new tools are needed to functionally investigate hundreds of putative essential protein coding genes. Accordingly, we recently implemented the auxin-inducible degron (AID) system for studying essential proteins in T. gondii. Here we provide a step-by-step protocol for examining protein function in T. gondii using the AID system in a tissue culture setting.

Entities:  

Keywords:  AID; Auxin; CRISPR; Conditional knockdown; Degron; Parasite; Protein regulation; Toxoplasma gondii

Year:  2018        PMID： 29644255      PMCID： PMC5890294          DOI： 10.21769/BioProtoc.2728

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  14 in total

1.  An auxin-based degron system for the rapid depletion of proteins in nonplant cells.

Authors:  Kohei Nishimura; Tatsuo Fukagawa; Haruhiko Takisawa; Tatsuo Kakimoto; Masato Kanemaki
Journal:  Nat Methods       Date:  2009-11-15       Impact factor: 28.547

2.  Development of CRISPR/Cas9 for Efficient Genome Editing in Toxoplasma gondii.

Authors:  Bang Shen; Kevin Brown; Shaojun Long; L David Sibley
Journal:  Methods Mol Biol       Date:  2017

3.  Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation.

Authors:  John G Doench; Ella Hartenian; Daniel B Graham; Zuzana Tothova; Mudra Hegde; Ian Smith; Meagan Sullender; Benjamin L Ebert; Ramnik J Xavier; David E Root
Journal:  Nat Biotechnol       Date:  2014-09-03       Impact factor: 54.908

4.  Transient transfection and expression in the obligate intracellular parasite Toxoplasma gondii.

Authors:  D Soldati; J C Boothroyd
Journal:  Science       Date:  1993-04-16       Impact factor: 47.728

5.  Aldolase is essential for energy production and bridging adhesin-actin cytoskeletal interactions during parasite invasion of host cells.

Authors:  G Lucas Starnes; Mathieu Coincon; Jurgen Sygusch; L David Sibley
Journal:  Cell Host Microbe       Date:  2009-04-23       Impact factor: 21.023

6.  Conditional Degradation of Plasmodium Calcineurin Reveals Functions in Parasite Colonization of both Host and Vector.

Authors:  Nisha Philip; Andrew P Waters
Journal:  Cell Host Microbe       Date:  2015-06-25       Impact factor: 21.023

7.  A conserved ankyrin repeat-containing protein regulates conoid stability, motility and cell invasion in Toxoplasma gondii.

Authors:  Shaojun Long; Bryan Anthony; Lisa L Drewry; L David Sibley
Journal:  Nat Commun       Date:  2017-12-21       Impact factor: 14.919

8.  2A and the auxin-based degron system facilitate control of protein levels in Plasmodium falciparum.

Authors:  Andrea Kreidenweiss; Annika V Hopkins; Benjamin Mordmüller
Journal:  PLoS One       Date:  2013-11-13       Impact factor: 3.240

9.  Efficient genome engineering of Toxoplasma gondii using CRISPR/Cas9.

Authors:  Saima M Sidik; Caroline G Hackett; Fanny Tran; Nicholas J Westwood; Sebastian Lourido
Journal:  PLoS One       Date:  2014-06-27       Impact factor: 3.240

10.  Efficient gene disruption in diverse strains of Toxoplasma gondii using CRISPR/CAS9.

Authors:  Bang Shen; Kevin M Brown; Tobie D Lee; L David Sibley
Journal:  mBio       Date:  2014-05-13       Impact factor: 7.867
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  22 in total

1.  Essential cGMP Signaling in Toxoplasma Is Initiated by a Hybrid P-Type ATPase-Guanylate Cyclase.

Authors:  Kevin M Brown; L David Sibley
Journal:  Cell Host Microbe       Date:  2018-11-15       Impact factor: 21.023

2.  Composition and organization of kinetochores show plasticity in apicomplexan chromosome segregation.

Authors:  Lorenzo Brusini; Nicolas Dos Santos Pacheco; Eelco C Tromer; Dominique Soldati-Favre; Mathieu Brochet
Journal:  J Cell Biol       Date:  2022-08-25       Impact factor: 8.077

3.  Screening the Toxoplasma kinome with high-throughput tagging identifies a regulator of invasion and egress.

Authors:  Tyler A Smith; Gabriella S Lopez-Perez; Alice L Herneisen; Emily Shortt; Sebastian Lourido
Journal:  Nat Microbiol       Date:  2022-04-28       Impact factor: 30.964

Review 4.  Paving the Way: Contributions of Big Data to Apicomplexan and Kinetoplastid Research.

Authors:  Robyn S Kent; Emma M Briggs; Beatrice L Colon; Catalina Alvarez; Sara Silva Pereira; Mariana De Niz
Journal:  Front Cell Infect Microbiol       Date:  2022-06-06       Impact factor: 6.073

5.  Restriction Checkpoint Controls Bradyzoite Development in Toxoplasma gondii.

Authors:  Anatoli V Naumov; Chengqi Wang; Dale Chaput; Li-Min Ting; Carmelo A Alvarez; Thomas Keller; Ahmed Ramadan; Michael W White; Kami Kim; Elena S Suvorova
Journal:  Microbiol Spectr       Date:  2022-06-02

6.  Three F-actin assembly centers regulate organelle inheritance, cell-cell communication and motility in Toxoplasma gondii.

Authors:  Nicolò Tosetti; Nicolas Dos Santos Pacheco; Dominique Soldati-Favre; Damien Jacot
Journal:  Elife       Date:  2019-02-12       Impact factor: 8.140

7.  CRISPR-mediated Tagging with BirA Allows Proximity Labeling in Toxoplasma gondii.

Authors:  Shaojun Long; Kevin M Brown; L David Sibley
Journal:  Bio Protoc       Date:  2018-03-20

8.  Coupling Auxin-Inducible Degron System with Ultrastructure Expansion Microscopy to Accelerate the Discovery of Gene Function in Toxoplasma gondii.

Authors:  Nicolas Dos Santos Pacheco; Dominique Soldati-Favre
Journal:  Methods Mol Biol       Date:  2021

9.  Rab11A regulates dense granule transport and secretion during Toxoplasma gondii invasion of host cells and parasite replication.

Authors:  Kannan Venugopal; Sylia Chehade; Elisabeth Werkmeister; Nicolas Barois; Javier Periz; Frank Lafont; Isabelle Tardieux; Jamal Khalife; Gordon Langsley; Markus Meissner; Sabrina Marion
Journal:  PLoS Pathog       Date:  2020-05-28       Impact factor: 6.823

10.  Essential function of the alveolin network in the subpellicular microtubules and conoid assembly in Toxoplasma gondii.

Authors:  Nicolò Tosetti; Nicolas Dos Santos Pacheco; Eloïse Bertiaux; Bohumil Maco; Lorène Bournonville; Virginie Hamel; Paul Guichard; Dominique Soldati-Favre
Journal:  Elife       Date:  2020-05-07       Impact factor: 8.140
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