Literature DB >> 2963757

A novel dinitrophenylglutathione-stimulated ATPase is present in human erythrocyte membranes.

E F LaBelle1, S V Singh, H Ahmad, L Wronski, S K Srivastava, Y C Awasthi.   

Abstract

Vesicles prepared from human erythrocyte membranes were found to catalyze ATP hydrolysis that was stimulated by dinitrophenylglutathione (Dnp-SG). This activity was dependent on temperature and Mg2+ and independent of ion pump ATPases present in erythrocyte membranes. The activity was a linear function of protein and time up to 60 min. The Km values of ATPase for Dnp-SG and ATP were found to be 49 microM and 1.67 mM, respectively. This suggests that in erythrocytes, the transport of Dnp-SG requires direct enzymatic hydrolysis of ATP and both Dnp-SG-stimulated ATPase activity and the ATP-dependent efflux of Dnp-SG from erythrocytes represent different activities of the same protein.

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Year:  1988        PMID： 2963757     DOI： 10.1016/0014-5793(88)80583-0

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  8 in total

1.  ATP dependent primary active transport of xenobiotic-glutathione conjugates by human erythrocyte membrane.

Authors:  Y C Awasthi; S V Singh; H Ahmad; L W Wronski; S K Srivastava; E F LaBelle
Journal:  Mol Cell Biochem       Date:  1989 Nov 23-Dec 19       Impact factor: 3.396

2.  ATP-dependent glutathione disulphide transport mediated by the MRP gene-encoded conjugate export pump.

Authors:  I Leier; G Jedlitschky; U Buchholz; M Center; S P Cole; R G Deeley; D Keppler
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Authors:  S K Srivastava; X Hu; H Xia; R J Bleicher; H A Zaren; J L Orchard; S Awasthi; S V Singh
Journal:  Biochem J       Date:  1998-06-15       Impact factor: 3.857

4.  Weakened cellular scavenging activity against oxidative stress in diabetes mellitus: regulation of glutathione synthesis and efflux.

Authors:  K Yoshida; J Hirokawa; S Tagami; Y Kawakami; Y Urata; T Kondo
Journal:  Diabetologia       Date:  1995-02       Impact factor: 10.122

Review 5.  RLIP76 and Cancer.

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6.  Adenosine triphosphate-dependent transport of doxorubicin, daunomycin, and vinblastine in human tissues by a mechanism distinct from the P-glycoprotein.

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7.  Organic anions exhibit distinct inhibition patterns on the low-Km and high-Km transport of S-(2,4-dinitrophenyl)glutathione through the human erythrocyte membrane.

Authors:  G Bartosz; H Sies; T P Akerboom
Journal:  Biochem J       Date:  1993-05-15       Impact factor: 3.857

8.  RALBP1 in Oxidative Stress and Mitochondrial Dysfunction in Alzheimer's Disease.

Authors:  Sanjay Awasthi; Ashly Hindle; Neha A Sawant; Mathew George; Murali Vijayan; Sudhir Kshirsagar; Hallie Morton; Lloyd E Bunquin; Philip T Palade; J Josh Lawrence; Hafiz Khan; Chhanda Bose; P Hemachandra Reddy; Sharda P Singh
Journal:  Cells       Date:  2021-11-10       Impact factor: 6.600
  8 in total

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