Tarek Kamal Abouzed1, María Del Mar Contreras2, Kadry Mohamed Sadek3, Moustafa Shukry4, Doaa H Abdelhady5, Wael Mohamed Gouda6, Walied Abdo7, Nasr Elsayed Nasr1, Reham Hassan Mekky8, Antonio Segura-Carretero2, Khaled Abdel-Aleim Kahilo1, Essam Abdel-Sattar9. 1. Department of Biochemistry, Faculty of Veterinary Medicine, Kafr-Elsheikh University, 33516 Kafr-Elsheikh, Egypt. 2. Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avenida Fuentenueva s/n, 18071 Granada, Spain; Research and Development Functional Food Centre (CIDAF), Bioregiόn Building, Health Science Technological Park, Avenida del Conocimiento s/n, 18016 Granada, Spain. 3. Department of Biochemistry, Faculty of Veterinary Medicine, Damanhur University, 22511 Damanhur, Egypt. 4. Department of Physiology, Faculty of Veterinary Medicine, Kafr-Elsheikh University, 33516 Kafr-Elsheikh, Egypt. 5. Department of Clinical Pathology, Faculty of Veterinary Medicine, Kafr-Elsheikh University, 33516 Kafr-Elsheikh, Egypt. 6. Department of Clinical Pathology, Faculty of Veterinary Medicine, Damanhur University, 22511 Damanhur, Egypt. 7. Department of Pathology, Faculty of Veterinary Medicine, Kafr-Elsheikh University, 33516 Kafr-Elsheikh, Egypt. 8. Research and Development Functional Food Centre (CIDAF), Bioregiόn Building, Health Science Technological Park, Avenida del Conocimiento s/n, 18016 Granada, Spain; Department of Pharmacognosy, Faculty of Pharmacy, Egyptian Russian University, Badr City, Cairo-Suez Road, 11829 Cairo, Egypt. 9. Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562 Cairo, Egypt. Electronic address: essam.abdelsattar@pharma.cu.edu.eg.
Abstract
AIM: The present study was designed to investigate the effect of red onion scales extract (ROS) against diabetic nephropathy, in relation to its metabolic profiling. METHODS: Four groups of male Wistar rats were assigned as follows; 1st untreated group, 2nd group (animals with diabetes) treated with streptozotocin (STZ, 50 mg/kg) IP, 3rd group co-treated with ROS (150 mg/kg + STZ, 50 mg/kg) and 4th group co-treated with ROS by a dose (300 mg/kg + STZ, 50 mg/kg) daily. After four weeks, random and fasting blood glucose (FBG) levels, serum insulin, advanced glycation end products (AGEs), urea, uric acid and inflammatory and fibrotic gene expression were evaluated. Moreover, histopathological examination of the renal tissues was performed. In addition, the metabolic profiling of ROS was performed via RP-HPLC-DAD-QTOF-MS and -MS/MS. RESULTS: The metabolic profiling of ROS revealed that protocatechuic acid and cyanidin-3-O-glucoside were the predominant compounds among 32 metabolites identified in the extract. ROS treated groups showed improvement of FBG and AGEs levels, whereas serum insulin level showed significant elevation. In addition, down-regulation of inflammatory mRNA expression associated with the hyperglycemic condition and amelioration in histopathological alterations in kidney tissues were observed. CONCLUSION: This study displayed the presence of 32 phenolic compounds in the ethanolic extract of ROS, a common by-product of the industrial production of onion in Egypt. This study proved the therapeutic potential of ROS as antidiabetic agent and its preventive effect against diabetic nephropathy. Therefore, this study represents a perspective of the utilization of food waste products.
AIM: The present study was designed to investigate the effect of red onion scales extract (ROS) against diabetic nephropathy, in relation to its metabolic profiling. METHODS: Four groups of male Wistar rats were assigned as follows; 1st untreated group, 2nd group (animals with diabetes) treated with streptozotocin (STZ, 50 mg/kg) IP, 3rd group co-treated with ROS (150 mg/kg + STZ, 50 mg/kg) and 4th group co-treated with ROS by a dose (300 mg/kg + STZ, 50 mg/kg) daily. After four weeks, random and fasting blood glucose (FBG) levels, serum insulin, advanced glycation end products (AGEs), urea, uric acid and inflammatory and fibrotic gene expression were evaluated. Moreover, histopathological examination of the renal tissues was performed. In addition, the metabolic profiling of ROS was performed via RP-HPLC-DAD-QTOF-MS and -MS/MS. RESULTS: The metabolic profiling of ROS revealed that protocatechuic acid and cyanidin-3-O-glucoside were the predominant compounds among 32 metabolites identified in the extract. ROS treated groups showed improvement of FBG and AGEs levels, whereas serum insulin level showed significant elevation. In addition, down-regulation of inflammatory mRNA expression associated with the hyperglycemic condition and amelioration in histopathological alterations in kidney tissues were observed. CONCLUSION: This study displayed the presence of 32 phenolic compounds in the ethanolic extract of ROS, a common by-product of the industrial production of onion in Egypt. This study proved the therapeutic potential of ROS as antidiabetic agent and its preventive effect against diabetic nephropathy. Therefore, this study represents a perspective of the utilization of food waste products.
Authors: Fatma M El-Demerdash; Yousra Talaat; Nora F Ghanem; Wenyi Kang Journal: Evid Based Complement Alternat Med Date: 2022-04-27 Impact factor: 2.650
Authors: Essam Abdel-Sattar; Engy A Mahrous; Mareena M Thabet; Dina M Yousry Elnaggar; Amal M Youssef; Reda Elhawary; Sawsan A Zaitone; Antonio Segura-Carretero; Reham Hassan Mekky Journal: Inflammopharmacology Date: 2020-10-28 Impact factor: 4.473