Claire Watkins1,2, Kiera Murphy1,2, Eugene M Dempsey3,4, Brendan P Murphy3,4, Paul W O'Toole2,5, R Paul Ross1,5, Catherine Stanton1,5, C Anthony Ryan6,7. 1. Teagasc Food Research Programme, Moorepark, Fermoy, Co. Cork, Ireland. 2. Department of Microbiology, University College Cork, Cork, Ireland. 3. Department of Paediatric and Child Health, Neonatology Department, Cork University Maternity Hospital, Cork, Ireland. 4. Department of Neonatology, Cork University Maternity Hospital, Wilton, Cork, Ireland. 5. APC Microbiome Ireland , Biosciences Institute, Cork, Ireland. 6. Department of Paediatric and Child Health, Neonatology Department, Cork University Maternity Hospital, Cork, Ireland. tony.ryan@ucc.ie. 7. Department of Neonatology, Cork University Maternity Hospital, Wilton, Cork, Ireland. tony.ryan@ucc.ie.
Abstract
The aim of this study was to determine bacteriological stability of a probiotic mixture dispersed in various diluents. The commercially available probiotic (Infloran®), containing Bifidobacterium bifidum (109 CFU/250 mg tablet) and Lactobacillus acidophilus (109 CFU/250 mg tablet), was dispersed within expressed breast milk, sterile water, and infant formula and examined at temperatures of 4 and 21 °C. When stored at 4 °C, significant decreases (P < 0.05) in the level of L. acidophilus and B. bifidum were observed in expressed breast milk and sterile water after a 6-h period. However, when stored in infant formula, both strains remained stable over a 12-h period. When stored at 21 °C, a significant decrease (P < 0.05) was observed in the level of L. acidophilus in sterile water, expressed breast milk and infant formula throughout a 12-h period. However, no significant decrease was observed overtime in B. bifidum in all three diluents at this temperature. CONCLUSION: Our findings suggest that, when stored at 4 °C, this probiotic product can remain at a stable condition for 6 h in sterile water and infant formula; however, the viability of the probiotic decreases significantly after this period of time. Administration of this probiotic in sterile water can be an acceptable alternative to dispersion and administration in expressed breast milk. What is Known: • Administration of probiotics containing lactobacilli and bifidobacteria has become more widespread in neonatology, mainly as prophylaxis for the prevention of necrotising entercolitis in preterm infants. • Probiotic reconstitution, from its powder base, is not standardized and various diluents, including sterile water, breast milk, and infant formula, have been used. What is New: • When stored at 4 °C, a probiotic containing lactobacilli and bifidobacteria remains at a stable microbological condition for up to 6 h in sterile water. • Administration of this probiotic dispersed in sterile water, followed by an EBM feed, can be an acceptable alternative to dispersion and administration in EBM.
The aim of this study was to determine bacteriological stability of a probiotic mixture dispersed in various diluents. The commercially available probiotic (Infloran®), containing Bifidobacterium bifidum (109 CFU/250 mg tablet) and Lactobacillus acidophilus (109 CFU/250 mg tablet), was dispersed within expressed breast milk, sterile water, and infant formula and examined at temperatures of 4 and 21 °C. When stored at 4 °C, significant decreases (P < 0.05) in the level of L. acidophilus and B. bifidum were observed in expressed breast milk and sterile water after a 6-h period. However, when stored in infant formula, both strains remained stable over a 12-h period. When stored at 21 °C, a significant decrease (P < 0.05) was observed in the level of L. acidophilus in sterile water, expressed breast milk and infant formula throughout a 12-h period. However, no significant decrease was observed overtime in B. bifidum in all three diluents at this temperature. CONCLUSION: Our findings suggest that, when stored at 4 °C, this probiotic product can remain at a stable condition for 6 h in sterile water and infant formula; however, the viability of the probiotic decreases significantly after this period of time. Administration of this probiotic in sterile water can be an acceptable alternative to dispersion and administration in expressed breast milk. What is Known: • Administration of probiotics containing lactobacilli and bifidobacteria has become more widespread in neonatology, mainly as prophylaxis for the prevention of necrotising entercolitis in preterm infants. • Probiotic reconstitution, from its powder base, is not standardized and various diluents, including sterile water, breast milk, and infant formula, have been used. What is New: • When stored at 4 °C, a probiotic containing lactobacilli and bifidobacteria remains at a stable microbological condition for up to 6 h in sterile water. • Administration of this probiotic dispersed in sterile water, followed by an EBM feed, can be an acceptable alternative to dispersion and administration in EBM.
Entities:
Keywords:
Expressed breast milk; Newborns; Preterm; Probiotics; Water
Authors: Nadja Larsen; Finn K Vogensen; Rikke Gøbel; Kim F Michaelsen; Waleed Abu Al-Soud; Søren J Sørensen; Lars H Hansen; Mogens Jakobsen Journal: FEMS Microbiol Ecol Date: 2011-01-11 Impact factor: 4.194
Authors: Yunwei Wang; Jeanette D Hoenig; Kathryn J Malin; Sanaa Qamar; Elaine O Petrof; Jun Sun; Dionysios A Antonopoulos; Eugene B Chang; Erika C Claud Journal: ISME J Date: 2009-04-16 Impact factor: 10.302