| Literature DB >> 29608556 |
Philipp Rosendahl1, Katarzyna Plak1,2, Angela Jacobi1, Martin Kraeter1,3, Nicole Toepfner1,4, Oliver Otto1, Christoph Herold1, Maria Winzi1, Maik Herbig1, Yan Ge1, Salvatore Girardo5, Katrin Wagner1, Buzz Baum2, Jochen Guck1.
Abstract
The throughput of cell mechanical characterization has recently approached that of conventional flow cytometers. However, this very sensitive, label-free approach still lacks the specificity of molecular markers. Here we developed an approach that combines real-time 1D-imaging fluorescence and deformability cytometry in one instrument (RT-FDC), thus opening many new research avenues. We demonstrated its utility by using subcellular fluorescence localization to identify mitotic cells and test for mechanical changes in those cells in an RNA interference screen.Entities:
Mesh：
Year: 2018 PMID： 29608556 DOI： 10.1038/nmeth.4639
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547