Literature DB >> 22426490

Tracking protein aggregation and mislocalization in cells with flow cytometry.

Yasmin M Ramdzan1, Saskia Polling, Cheryl P Z Chia, Ivan H W Ng, Angelique R Ormsby, Nathan P Croft, Anthony W Purcell, Marie A Bogoyevitch, Dominic C H Ng, Paul A Gleeson, Danny M Hatters.   

Abstract

We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington's disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.

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Year:  2012        PMID： 22426490     DOI： 10.1038/nmeth.1930

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  17 in total

1.  Tracking mutant huntingtin aggregation kinetics in cells reveals three major populations that include an invariant oligomer pool.

Authors:  Maya A Olshina; Lauren M Angley; Yasmin M Ramdzan; Jinwei Tang; Michael F Bailey; Andrew F Hill; Danny M Hatters
Journal:  J Biol Chem       Date:  2010-05-05       Impact factor: 5.157

2.  High content image cytometry in the context of subnuclear organization.

Authors:  W H De Vos; L Van Neste; B Dieriks; G H Joss; P Van Oostveldt
Journal:  Cytometry A       Date:  2010-01       Impact factor: 4.355

3.  Retrograde transport of KDEL-bearing B-fragment of Shiga toxin.

Authors:  L Johannes; D Tenza; C Antony; B Goud
Journal:  J Biol Chem       Date:  1997-08-01       Impact factor: 5.157

4.  A novel gene containing a trinucleotide repeat that is expanded and unstable on Huntington's disease chromosomes. The Huntington's Disease Collaborative Research Group.

Authors: 
Journal:  Cell       Date:  1993-03-26       Impact factor: 41.582

5.  A two-step path to inclusion formation of huntingtin peptides revealed by number and brightness analysis.

Authors:  Giulia Ossato; Michelle A Digman; Charity Aiken; Tamas Lukacsovich; J Lawrence Marsh; Enrico Gratton
Journal:  Biophys J       Date:  2010-06-16       Impact factor: 4.033

6.  Amyotrophic lateral sclerosis and structural defects in Cu,Zn superoxide dismutase.

Authors:  H X Deng; A Hentati; J A Tainer; Z Iqbal; A Cayabyab; W Y Hung; E D Getzoff; P Hu; B Herzfeldt; R P Roos
Journal:  Science       Date:  1993-08-20       Impact factor: 47.728

Review 7.  Protein misfolding inside cells: the case of huntingtin and Huntington's disease.

Authors:  Danny M Hatters
Journal:  IUBMB Life       Date:  2008-11       Impact factor: 3.885

8.  Inherent toxicity of aggregates implies a common mechanism for protein misfolding diseases.

Authors:  Monica Bucciantini; Elisa Giannoni; Fabrizio Chiti; Fabiana Baroni; Lucia Formigli; Jesús Zurdo; Niccolò Taddei; Giampietro Ramponi; Christopher M Dobson; Massimo Stefani
Journal:  Nature       Date:  2002-04-04       Impact factor: 49.962

9.  Formation and toxicity of soluble polyglutamine oligomers in living cells.

Authors:  Patrick Lajoie; Erik Lee Snapp
Journal:  PLoS One       Date:  2010-12-28       Impact factor: 3.240

10.  Aggresomes: a cellular response to misfolded proteins.

Authors:  J A Johnston; C L Ward; R R Kopito
Journal:  J Cell Biol       Date:  1998-12-28       Impact factor: 10.539
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  44 in total

Review 1.  Cargo trafficking between endosomes and the trans-Golgi network.

Authors:  Pei Zhi Cheryl Chia; Priscilla Gunn; Paul A Gleeson
Journal:  Histochem Cell Biol       Date:  2013-07-14       Impact factor: 4.304

2.  Modulation of Fluorescent Protein Chromophores To Detect Protein Aggregation with Turn-On Fluorescence.

Authors:  Yu Liu; Charles H Wolstenholme; Gregory C Carter; Hongbin Liu; Hang Hu; Leeann S Grainger; Kun Miao; Matthew Fares; Conner A Hoelzel; Hemant P Yennawar; Gang Ning; Manyu Du; Lu Bai; Xiaosong Li; Xin Zhang
Journal:  J Am Chem Soc       Date:  2018-06-12       Impact factor: 15.419

3.  Flow cytometric quantification and characterization of intracellular protein aggregates in yeast.

Authors:  Ayala Shiber; William Breuer; Tommer Ravid
Journal:  Prion       Date:  2014       Impact factor: 3.931

Review 4.  Physicochemical properties of cells and their effects on intrinsically disordered proteins (IDPs).

Authors:  Francois-Xavier Theillet; Andres Binolfi; Tamara Frembgen-Kesner; Karan Hingorani; Mohona Sarkar; Ciara Kyne; Conggang Li; Peter B Crowley; Lila Gierasch; Gary J Pielak; Adrian H Elcock; Anne Gershenson; Philipp Selenko
Journal:  Chem Rev       Date:  2014-06-05       Impact factor: 60.622

5.  Ubiquilin-2 differentially regulates polyglutamine disease proteins.

Authors:  Julia E Gerson; Nathaniel Safren; Svetlana Fischer; Ronak Patel; Emily V Crowley; Jacqueline P Welday; Alexandra K Windle; Sami Barmada; Henry L Paulson; Lisa M Sharkey
Journal:  Hum Mol Genet       Date:  2020-08-29       Impact factor: 6.150

6.  AggFluor: Fluorogenic Toolbox Enables Direct Visualization of the Multi-Step Protein Aggregation Process in Live Cells.

Authors:  Charles H Wolstenholme; Hang Hu; Songtao Ye; Brian E Funk; Divya Jain; Chia-Heng Hsiung; Gang Ning; Yu Liu; Xiaosong Li; Xin Zhang
Journal:  J Am Chem Soc       Date:  2020-10-05       Impact factor: 15.419

7.  The aggregation and inheritance of damaged proteins determines cell fate during mitosis.

Authors:  Mary Rose Bufalino; Derek van der Kooy
Journal:  Cell Cycle       Date:  2014-02-11       Impact factor: 4.534

Review 8.  Chemical Biology Framework to Illuminate Proteostasis.

Authors:  Rebecca M Sebastian; Matthew D Shoulders
Journal:  Annu Rev Biochem       Date:  2020-02-25       Impact factor: 23.643

9.  Polyalanine expansions drive a shift into α-helical clusters without amyloid-fibril formation.

Authors:  Saskia Polling; Angelique R Ormsby; Rebecca J Wood; Kristie Lee; Cheryl Shoubridge; James N Hughes; Paul Q Thomas; Michael D W Griffin; Andrew F Hill; Quill Bowden; Till Böcking; Danny M Hatters
Journal:  Nat Struct Mol Biol       Date:  2015-11-16       Impact factor: 15.369

10.  Misfolded polyglutamine, polyalanine, and superoxide dismutase 1 aggregate via distinct pathways in the cell.

Authors:  Saskia Polling; Yee-Foong Mok; Yasmin M Ramdzan; Bradley J Turner; Justin J Yerbury; Andrew F Hill; Danny M Hatters
Journal:  J Biol Chem       Date:  2014-01-14       Impact factor: 5.157
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