Literature DB >> 2959795

Dissociation of NS5 from cell fractions containing West Nile virus-specific polymerase activity.

J B Grun1, M A Brinton.   

Abstract

West Nile virus replication complexes were partially purified from cytoplasmic extracts of virus-infected cells by centrifugation through a 20% glycerol cushion. Numerous cell proteins, as well as the largest nonstructural protein, NS5, were separated from the replication complexes without significant loss of in vitro West Nile virus polymerase activity.

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Year:  1987        PMID： 2959795      PMCID： PMC255969     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  24 in total

1.  Phage Q-beta ribonucleic acid replicase. Subunit relationships determined by intramolecular cross-linking.

Authors:  R A Young; T Blumenthal
Journal:  J Biol Chem       Date:  1975-03-10       Impact factor: 5.157

2.  Purification and polypeptide composition of Semliki Forest virus RNA polymerase.

Authors:  J P Clewley; S I Kennedy
Journal:  J Gen Virol       Date:  1976-09       Impact factor: 3.891

3.  Qbeta RNA replicase and protein synthesis elongation factors EF-Tu and EF-Ts.

Authors:  T Blumenthal
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

4.  Function and structure in ribonucleic acid phage Q beta ribonucleic acid replicase. The roles of the different subunits in transcription of synthetic templates.

Authors:  T A Landers; T Blumenthal; K Weber
Journal:  J Biol Chem       Date:  1974-09-25       Impact factor: 5.157

5.  Autoradiographic localization of RNA synthesis directed by arboviruses in the cytoplasm of infected BHK-21 cells.

Authors:  A S Lubiniecki; C J Henry
Journal:  Proc Soc Exp Biol Med       Date:  1974-04

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Synthesis of alphavirus-specified RNA.

Authors:  H Brzeski; S I Kennedy
Journal:  J Virol       Date:  1978-02       Impact factor: 5.103

8.  Solubilized RNA replication complex from Semliki Forest virus-infected cells.

Authors:  M Ranki; L Kääriäinen
Journal:  Virology       Date:  1979-10-30       Impact factor: 3.616

9.  Characterization of West Nile virus RNA-dependent RNA polymerase and cellular terminal adenylyl and uridylyl transferases in cell-free extracts.

Authors:  J B Grun; M A Brinton
Journal:  J Virol       Date:  1986-12       Impact factor: 5.103

10.  Mechanism for control of synthesis of Semliki Forest virus 26S and 42s RNA.

Authors:  D L Sawicki; L Kaariainen; C Lambek; P J Gomatos
Journal:  J Virol       Date:  1978-01       Impact factor: 5.103
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  27 in total

1.  Molecular and ultrastructural analysis of heavy membrane fractions associated with the replication of Kunjin virus RNA.

Authors:  P W Chu; E G Westaway
Journal:  Arch Virol       Date:  1992       Impact factor: 2.574

2.  Construction and characterization of chimeric tick-borne encephalitis/dengue type 4 viruses.

Authors:  A G Pletnev; M Bray; J Huggins; C J Lai
Journal:  Proc Natl Acad Sci U S A       Date:  1992-11-01       Impact factor: 11.205

3.  Both nonstructural proteins NS2B and NS3 are required for the proteolytic processing of dengue virus nonstructural proteins.

Authors:  B Falgout; M Pethel; Y M Zhang; C J Lai
Journal:  J Virol       Date:  1991-05       Impact factor: 5.103

Review 4.  Flavivirus RNA synthesis in vitro.

Authors:  Radhakrishnan Padmanabhan; Ratree Takhampunya; Tadahisa Teramoto; Kyung H Choi
Journal:  Methods       Date:  2015-08-10       Impact factor: 3.608

5.  Interaction of TIA-1/TIAR with West Nile and dengue virus products in infected cells interferes with stress granule formation and processing body assembly.

Authors:  Mohamed M Emara; Margo A Brinton
Journal:  Proc Natl Acad Sci U S A       Date:  2007-05-14       Impact factor: 11.205

6.  CRM1-mediated nuclear export of dengue virus RNA polymerase NS5 modulates interleukin-8 induction and virus production.

Authors:  Stephen M Rawlinson; Melinda J Pryor; Peter J Wright; David A Jans
Journal:  J Biol Chem       Date:  2009-03-18       Impact factor: 5.157

7.  Maturation of dengue virus nonstructural protein 4B in monocytes enhances production of dengue hemorrhagic fever-associated chemokines and cytokines.

Authors:  James F Kelley; Pakieli H Kaufusi; Esther M Volper; Vivek R Nerurkar
Journal:  Virology       Date:  2011-08-02       Impact factor: 3.616

8.  Ultrastructure of Kunjin virus-infected cells: colocalization of NS1 and NS3 with double-stranded RNA, and of NS2B with NS3, in virus-induced membrane structures.

Authors:  E G Westaway; J M Mackenzie; M T Kenney; M K Jones; A A Khromykh
Journal:  J Virol       Date:  1997-09       Impact factor: 5.103

9.  Flavivirus infection: essential ultrastructural changes and association of Kunjin virus NS3 protein with microtubules.

Authors:  M L Ng; S S Hong
Journal:  Arch Virol       Date:  1989       Impact factor: 2.574

10.  Dengue type 4 virus mutants containing deletions in the 3' noncoding region of the RNA genome: analysis of growth restriction in cell culture and altered viremia pattern and immunogenicity in rhesus monkeys.

Authors:  R Men; M Bray; D Clark; R M Chanock; C J Lai
Journal:  J Virol       Date:  1996-06       Impact factor: 5.103
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