| Literature DB >> 29594378 |
Gang Liang1,2,3,4, Yan Man1,2,3, An Li1,2,3, Xinxin Jin1,2,3, Ligang Pan5,6,7, Xinhui Liu8.
Abstract
A chemiluminescence (CL) based assay is described for the determination of the environmental pollutant 2-hydroxyfluorene (2-HOFlu) which is found to inhibit the CL of a system composed of the G-quadruplex/hemin complex (a DNAzyme), H2O2, and luminol. The G-rich aptamer PW17 is transformed to a potassium(I)-stabilized G-quadruplex-hemin complex which displays peroxidase-like activity to catalyze the oxidation of luminol by H2O2 which is accompanied by strong blue CL emission. On addition of 2-HOFlu, it will participate in the G-quadruplex DNAzyme-mediated oxidation by H2O2. As a result, CL intensity is decreased. The difference in CL intensity (ΔI) before and after addition of 2-HOFlu serves as the signal for its quantitation. In water of pH 9.0, a linear relationship is found for the 1 nM to 1 μM concentration range, with a 0.2 nM detection limit. The assay is highly selective over other fluorene derivatives. It was successfully applied to the determination of 2-HOFlu in spiked lake water samples. The method is rapid, cost-effective and convenient. Conceivably, it has a wide scope in that it may be applied to other target pollutants for which G-quadruplexes are available. Graphical abstract A chemiluminescence (CL) assay is described for the determination of the environmental pollutant 2-hydroxyfluorene (2-HOFlu) based on the inhibition of the CL system composed of the G-quadruplex/hemin complex (a DNAzyme), H2O2, and luminol.Entities:
Keywords: Aptamer; Environmental contaminants; Fluorene; G-rich deoxyribonucleic acids; HRP-mimicking DNAzyme; Hydroxyfluorenen; Polycyclic aromatic hydrocarbons; Quantitation; Selective; Sensing probe
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Year: 2017 PMID： 29594378 DOI： 10.1007/s00604-017-2555-y
Source DB: PubMed Journal: Mikrochim Acta ISSN： 0026-3672 Impact factor: 5.833