Tian Tian1, Linlin Wang1, Yan Shen2, Bo Zhang2, Richard H Finnell3, Aiguo Ren1. 1. Institute of Reproductive & Child Health, Ministry of Health Key Laboratory of Reproductive Health, Department of Epidemiology & Biostatistics, School of Public Health, Peking University Health Science Center, Beijing, PR China. 2. Key Laboratory of Cell Proliferation & Differentiation of Ministry of Education, College of Life Sciences, Peking University, Beijing, PR China. 3. Departments of Molecular & Cellular Biology & Medicine, Baylor College of Medicine, Houston, TX 77030, USA.
Abstract
AIM: To investigate the relationship between GRHL3 methylation and the etiology of neural tube defects (NTDs). MATERIALS & METHODS: Analyze data from a genome-wide DNA methylation array. Targeted DNA methylation analysis was performed for 46 cases and 23 controls. At last, grhl3 overexpression and gene depletion experiments were conducted in zebrafish. RESULTS: Five hypomethylated CpGs were discovered in the methylation arrays performed on NTD cases. In a validation study, 15 hypomethylated CpGs were found and the overall methylation levels decreased in brain/spinal cord tissue from NTD cases. The knockdown and overexpression of grhl3 in zebrafish damaged embryonic convergent extension processes. CONCLUSION: Hypomethylation of GRHL3 in central nervous tissue is associated with NTDs, further supporting the importance of GRHL3 and methylation in proper neural tube closure.
AIM: To investigate the relationship between GRHL3 methylation and the etiology of neural tube defects (NTDs). MATERIALS & METHODS: Analyze data from a genome-wide DNA methylation array. Targeted DNA methylation analysis was performed for 46 cases and 23 controls. At last, grhl3 overexpression and gene depletion experiments were conducted in zebrafish. RESULTS: Five hypomethylated CpGs were discovered in the methylation arrays performed on NTD cases. In a validation study, 15 hypomethylated CpGs were found and the overall methylation levels decreased in brain/spinal cord tissue from NTD cases. The knockdown and overexpression of grhl3 in zebrafish damaged embryonic convergent extension processes. CONCLUSION: Hypomethylation of GRHL3 in central nervous tissue is associated with NTDs, further supporting the importance of GRHL3 and methylation in proper neural tube closure.
Authors: Philippe Lemay; Patrizia De Marco; Alexandre Emond; Dan Spiegelman; Alexandre Dionne-Laporte; Sandra Laurent; Elisa Merello; Andrea Accogli; Guy A Rouleau; Valeria Capra; Zoha Kibar Journal: Hum Mutat Date: 2017-03-24 Impact factor: 4.878
Authors: Sebastian Dworkin; Johanna Simkin; Charbel Darido; Darren D Partridge; Smitha R Georgy; Jacinta Caddy; Tomasz Wilanowski; Graham J Lieschke; Karen Doggett; Joan K Heath; Stephen M Jane Journal: Mech Dev Date: 2014-06-07 Impact factor: 1.882
Authors: Sanja A Farkas; Anna K Böttiger; Helena S Isaksson; Richard H Finnell; Aiguo Ren; Torbjörn K Nilsson Journal: Epigenetics Date: 2013-02-15 Impact factor: 4.528
Authors: Jemma G Gasperoni; Jarrad N Fuller; Charbel Darido; Tomasz Wilanowski; Sebastian Dworkin Journal: Int J Mol Sci Date: 2022-03-01 Impact factor: 6.208
Authors: Sandra C P De Castro; Peter Gustavsson; Abigail R Marshall; William M Gordon; Gabriel Galea; Evanthia Nikolopoulou; Dawn Savery; Ana Rolo; Philip Stanier; Bogi Andersen; Andrew J Copp; Nicholas D E Greene Journal: Hum Mol Genet Date: 2018-12-15 Impact factor: 6.150