Literature DB >> 29577990

Whole-genome sequencing in the investigation of recurrent invasive group A streptococcus outbreaks in a maternity unit.

H Dickinson1, M Reacher1, B Nazareth2, H Eagle3, D Fowler3, A Underwood4, M Chand5, V Chalker4, J Coelho4, R Daniel4, G Kapatai1, A Al-Shabib4, R Puleston6.   

Abstract

BACKGROUND: The clinical manifestations of group A streptococcus (GAS) (Streptococcus pyogenes) are diverse, ranging from asymptomatic colonization to devastating invasive disease. Maternity-related clusters of invasive GAS (iGAS) infection are complex to investigate and control, especially if recurrent. AIM: To investigate three episodes of emm 75 GAS/iGAS infection in maternity patients at one hospital site over a four-year period (two with monophyletic ancestry).
METHODS: The episodes are described, together with whole-genome sequence (WGS) isolate analyses. Single nucleotide polymorphism differences were compared with contemporaneous emm 75 genomes.
FINDINGS: Over the four-year study period, seven mothers had emm 75 GAS/iGAS and one mother had emm 3 iGAS (in year 4) (subsequently discounted as linked). Three (clinical/screening samples) of the seven babies of emm-75-positive mothers and three screened healthcare workers were positive for emm 75 GAS. WGS similarity suggested a shared ancestral lineage and a common source transmission, but directionality of transmission cannot be inferred. However, the findings indicate that persistence of a particular clone in a given setting may be long term.
CONCLUSIONS: Occupational health procedures were enhanced, staff were screened, and antibiotic therapy was provided to GAS-positive staff and patients. The definitive source of infection could not be identified, although staff-patient transmission was the most likely route. The pattern of clonal GAS transmission over the four-year study period suggests that long-term persistence of GAS may have occurred.
Copyright © 2018. Published by Elsevier Ltd.

Entities:  

Keywords:  Maternity; Streptococcus spp.; Whole-genome sequencing; iGAS

Mesh:

Year:  2018        PMID: 29577990     DOI: 10.1016/j.jhin.2018.03.018

Source DB:  PubMed          Journal:  J Hosp Infect        ISSN: 0195-6701            Impact factor:   3.926


  2 in total

1.  Three sequential outbreaks of Group A Streptococcus over a two-year period at the Canadian Forces Leadership and Recruit School, St. Jean Garrison, Québec.

Authors:  Barbara Strauss; Martin Tepper; Diane Lu; François Gagnon; Eric Girard; Walter Demczuk; Irene Martin; Martine Massé; Kirsten Barnes
Journal:  Can Commun Dis Rep       Date:  2020-09-03

2.  Restricted Sequence Variation in Streptococcus pyogenes Penicillin Binding Proteins.

Authors:  Andrew Hayes; Jake A Lacey; Jacqueline M Morris; Mark R Davies; Steven Y C Tong
Journal:  mSphere       Date:  2020-04-29       Impact factor: 4.389

  2 in total

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