Yichen Ding1,2, Juhyun Lee2,3, Jeffrey J Hsu1, Chih-Chiang Chang2, Kyung In Baek2, Sara Ranjbarvaziri1, Reza Ardehali1, René R Sevag Packard1, Tzung K Hsiai4,5,6. 1. Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, 90095, USA. 2. Department of Bioengineering, University of California, Los Angeles, CA, 90095, USA. 3. Department of Bioengineering, University of Texas at Arlington, Arlington, TX, 76010, USA. 4. Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, 90095, USA. Thsiai@mednet.ucla.edu. 5. Department of Bioengineering, University of California, Los Angeles, CA, 90095, USA. Thsiai@mednet.ucla.edu. 6. Medical Engineering, California Institute of Technology, Pasadena, CA, 91106, USA. Thsiai@mednet.ucla.edu.
Abstract
PURPOSE OF REVIEW: Real-time 3-dimensional (3-D) imaging of cardiovascular injury and regeneration remains challenging. We introduced a multi-scale imaging strategy that uses light-sheet illumination to enable applications of cardiovascular injury and repair in models ranging from zebrafish to rodent hearts. RECENT FINDINGS: Light-sheet imaging enables rapid data acquisition with high spatiotemporal resolution and with minimal photo-bleaching or photo-toxicity. We demonstrated the capacity of this novel light-sheet approach for scanning a region of interest with specific fluorescence contrast, thereby providing axial and temporal resolution at the cellular level without stitching image columns or pivoting illumination beams during one-time imaging. This cutting-edge imaging technique allows for elucidating the differentiation of stem cells in cardiac regeneration, providing an entry point to discover novel micro-circulation phenomenon with clinical significance for injury and repair. These findings demonstrate the multi-scale applications of this novel light-sheet imaging strategy to advance research in cardiovascular development and regeneration.
PURPOSE OF REVIEW: Real-time 3-dimensional (3-D) imaging of cardiovascular injury and regeneration remains challenging. We introduced a multi-scale imaging strategy that uses light-sheet illumination to enable applications of cardiovascular injury and repair in models ranging from zebrafish to rodent hearts. RECENT FINDINGS: Light-sheet imaging enables rapid data acquisition with high spatiotemporal resolution and with minimal photo-bleaching or photo-toxicity. We demonstrated the capacity of this novel light-sheet approach for scanning a region of interest with specific fluorescence contrast, thereby providing axial and temporal resolution at the cellular level without stitching image columns or pivoting illumination beams during one-time imaging. This cutting-edge imaging technique allows for elucidating the differentiation of stem cells in cardiac regeneration, providing an entry point to discover novel micro-circulation phenomenon with clinical significance for injury and repair. These findings demonstrate the multi-scale applications of this novel light-sheet imaging strategy to advance research in cardiovascular development and regeneration.
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