Erland Jørn Erlandsen1, Else Randers2. 1. Department of Clinical Biochemistry, Viborg Regional Hospital, Viborg, Denmark. 2. Department of Internal Medicine, Viborg Regional Hospital, Viborg, Denmark.
Abstract
INTRODUCTION: The aim of this study was to establish reference intervals for plasma cystatin C and creatinine in adults using the Gentians cystatin C method traceable to the international calibrator standard ERM-DA471/IFCC and a creatinine method traceable to the IDMS (Isotope Dilution Mass Spectrometry) creatinine reference method. METHODS: Blood samples were collected from 304 healthy blood donors (152 men and 152 women between 17 and 66 years old) with 30-31 men and 30-31 women in each ten-year interval. Plasma cystatin C was analyzed using the Gentian Cystatin C assay on a Roche cobas c702 analyzer, and plasma creatinine was analyzed using the CREA Plus assay on the Roche Modular P analyzer. RESULTS: The nonparametric reference intervals for plasma cystatin C were 0.58-1.00 mg/L in women (median 0.78 mg/L, range 0.56-1.06 mg/L) and 0.62-1.04 mg/L in men (median 0.79 mg/L, range 0.61-1.07 mg/L). The Mann-Whitney U test revealed no gender-related difference in plasma cystatin C (P = .21). A common reference interval in women and men was calculated to be 0.61-1.01 mg/L (median 0.79 mg/L, range 0.56-1.07 mg/L). The nonparametric reference interval for plasma creatinine was 52-89 μmol/L in women (median 69 μmol/L, range 52-92 μmol/L) and 61-108 μmol/L in men (median 86 μmol/L, range 56-118 μmol/L). The Mann-Whitney U test revealed a gender-related difference in plasma creatinine (P < .0001). CONCLUSION: In conclusion, we have established reference intervals for plasma cystatin C and creatinine in adults using methods traceable to international standards.
INTRODUCTION: The aim of this study was to establish reference intervals for plasma cystatin C and creatinine in adults using the Gentians cystatin C method traceable to the international calibrator standard ERM-DA471/IFCC and a creatinine method traceable to the IDMS (Isotope Dilution Mass Spectrometry) creatinine reference method. METHODS: Blood samples were collected from 304 healthy blood donors (152 men and 152 women between 17 and 66 years old) with 30-31 men and 30-31 women in each ten-year interval. Plasma cystatin C was analyzed using the Gentian Cystatin C assay on a Roche cobas c702 analyzer, and plasma creatinine was analyzed using the CREA Plus assay on the Roche Modular P analyzer. RESULTS: The nonparametric reference intervals for plasma cystatin C were 0.58-1.00 mg/L in women (median 0.78 mg/L, range 0.56-1.06 mg/L) and 0.62-1.04 mg/L in men (median 0.79 mg/L, range 0.61-1.07 mg/L). The Mann-Whitney U test revealed no gender-related difference in plasma cystatin C (P = .21). A common reference interval in women and men was calculated to be 0.61-1.01 mg/L (median 0.79 mg/L, range 0.56-1.07 mg/L). The nonparametric reference interval for plasma creatinine was 52-89 μmol/L in women (median 69 μmol/L, range 52-92 μmol/L) and 61-108 μmol/L in men (median 86 μmol/L, range 56-118 μmol/L). The Mann-Whitney U test revealed a gender-related difference in plasma creatinine (P < .0001). CONCLUSION: In conclusion, we have established reference intervals for plasma cystatin C and creatinine in adults using methods traceable to international standards.
Authors: Lesley A Inker; Christopher H Schmid; Hocine Tighiouart; John H Eckfeldt; Harold I Feldman; Tom Greene; John W Kusek; Jane Manzi; Frederick Van Lente; Yaping Lucy Zhang; Josef Coresh; Andrew S Levey Journal: N Engl J Med Date: 2012-07-05 Impact factor: 91.245
Authors: Robert C Kalayjian; Kevin R Robertson; Jeffrey M Albert; Carl J Fichtenbaum; Todd T Brown; Babafemi O Taiwo Journal: J Acquir Immune Defic Syndr Date: 2019-06-01 Impact factor: 3.731