Hexanal as a QS inhibitor of extracellular enzyme activity of Erwinia carotovora and Pseudomonas fluorescens and its application in vegetables.

To prevent the postharvest disease of Chinese cabbage and lettuce, hexanal was used as a control measure to inhibit N-acyl homoserine lactone (AHL) production and extracellular enzymes regulated by quorum-sensing (QS) in their main spoilage strains of Erwinia carotovora and Pseudomonas fluorescens. Firstly, the QS inhibition of hexanal was verified by significantly inhibiting violacein production (p < 0.05) in Chromobacterium violaceum CV026 at sub-MICs. β-Galactosidase activities which reflected AHL production, were significantly inhibited by hexanal, its inhibitory effect was concentration-dependent under minimal inhibitory concentration (MIC) (p < 0.05). The detected extracellular enzymes activities decreased with the increase of hexanal concentration (p < 0.05), including cellulase, xylanase, pectate lyase, polygalacturonase, and protease. Chinese cabbage soft rot and lettuce leaf scorch could be significantly inhibited by hexanal (p < 0.05) without any phytotoxicity effect, the 1/2 MIC of hexanal showed the best inhibitory effect. And all the above effects showed a dose-dependent. A novel preservation technique in reducing the loss of vegetables due to spoilage based on the QS inhibitor was developed.