Salvatore Sauro1, Ashvin Babbar2, Borzo Gharibi2, Victor Pinheiro Feitosa3, Ricardo Marins Carvalho4, Lidiany Karla Azevedo Rodrigues5, Avijit Banerjee6, Timothy Watson6. 1. Dental Biomaterials and Minimally Invasive Dentistry, Departmento de Odontologia, Facultad de Ciencias de la Salud, CEU-Cardenal Herrera University, Alfara del Patriarca, 46115 Valencia, Spain; Tissue Engineering and Biophotonics Research Division, King's College London Dental Institute, King's Health Partners, London, UK. Electronic address: salvatore.sauro@uch.ceu.es. 2. Tissue Engineering and Biophotonics Research Division, King's College London Dental Institute, King's Health Partners, London, UK. 3. School of Dentistry, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, Brazil; Paulo Picanço School of Dentistry, Fortaleza, Brazil. 4. Department of Oral Biological and Medical Sciences, Division of Biomaterials, Faculty of Dentistry, The University of British Columbia, Vancouver, BC V6T 1Z3, Canada. 5. School of Dentistry, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, Brazil. 6. Tissue Engineering and Biophotonics Research Division, King's College London Dental Institute, King's Health Partners, London, UK; Department of Conservative & MI Dentistry, King's College London Dental Institute, King's Health Partners, London, UK.
Abstract
OBJECTIVE: Materials for pulp protection should have therapeutic properties in order to stimulate remineralization and pulp reparative processes. The aim of this study was to evaluate the mechanical properties, biocompatibility, cell differentiation and bioactivity of experimental light-curable resin-based materials containing bioactive micro-fillers. METHODS: Four calcium-phosphosilicate micro-fillers were prepared and incorporated into a resin blend: 1) Bioglass 45S5 (BAG); 2) zinc-doped bioglass (BAG-Zn); 3) βTCP-modified calcium silicate (β-CS); 4) zinc-doped β-CS (β-CS-Zn). These experimental resins were tested for flexural strength (FS) and fracture toughness (FT) after 24h and 30-day storage in simulated body fluid (SBF). Cytotoxicity was evaluated using MTT assay, while bioactivity was evaluated using mineralization and gene expression assays (Runx-2 & ALP). RESULTS: The lowest FS and FT at 24h was attained with β-CS resin, while all the other tested materials exhibited a decrease in FS after prolonged storage in SBF. β-CS-Zn maintained a stable FT after 30-day SBF aging. Incorporation of bioactive micro-fillers had no negative effect on the biocompatibility of the experimental materials tested in this study. The inclusion of zinc-doped fillers significantly increased the cellular remineralization potential and expression of the osteogenic genes Runx2 and ALP (p<0.05). SIGNIFICANCE: The innovative materials tested in this study, in particular those containing β-CS-Zn and BAG-Zn may promote cell differentiation and mineralization. Thus, these materials might represent suitable therapeutic pulp protection materials for minimally invasive and atraumatic restorative treatments.
OBJECTIVE: Materials for pulp protection should have therapeutic properties in order to stimulate remineralization and pulp reparative processes. The aim of this study was to evaluate the mechanical properties, biocompatibility, cell differentiation and bioactivity of experimental light-curable resin-based materials containing bioactive micro-fillers. METHODS: Four calcium-phosphosilicate micro-fillers were prepared and incorporated into a resin blend: 1) Bioglass 45S5 (BAG); 2) zinc-doped bioglass (BAG-Zn); 3) βTCP-modified calcium silicate (β-CS); 4) zinc-doped β-CS (β-CS-Zn). These experimental resins were tested for flexural strength (FS) and fracture toughness (FT) after 24h and 30-day storage in simulated body fluid (SBF). Cytotoxicity was evaluated using MTT assay, while bioactivity was evaluated using mineralization and gene expression assays (Runx-2 & ALP). RESULTS: The lowest FS and FT at 24h was attained with β-CS resin, while all the other tested materials exhibited a decrease in FS after prolonged storage in SBF. β-CS-Zn maintained a stable FT after 30-day SBF aging. Incorporation of bioactive micro-fillers had no negative effect on the biocompatibility of the experimental materials tested in this study. The inclusion of zinc-doped fillers significantly increased the cellular remineralization potential and expression of the osteogenic genes Runx2 and ALP (p<0.05). SIGNIFICANCE: The innovative materials tested in this study, in particular those containing β-CS-Zn and BAG-Zn may promote cell differentiation and mineralization. Thus, these materials might represent suitable therapeutic pulp protection materials for minimally invasive and atraumatic restorative treatments.
Authors: Francisco Javier Rodríguez-Lozano; S López-García; D García-Bernal; J L Sanz; A Lozano; M P Pecci-Lloret; M Melo; C López-Ginés; L Forner Journal: Clin Oral Investig Date: 2021-02-27 Impact factor: 3.573
Authors: Sergio López-García; María P Pecci-Lloret; Miguel R Pecci-Lloret; Ricardo E Oñate-Sánchez; David García-Bernal; Pablo Castelo-Baz; Francisco Javier Rodríguez-Lozano; Julia Guerrero-Gironés Journal: Materials (Basel) Date: 2019-11-08 Impact factor: 3.623