Paula Maciel Pires1,2, Andrei Cristian Ionescu3, Maria Teresa Pérez-Gracia4, Elena Vezzoli5, Igor Paulino Mendes Soares6, Eugenio Brambilla3, Aline de Almeida Neves1, Salvatore Sauro7. 1. Department of Pediatric Dentistry, Universidade Federal Do Rio de Janeiro, Rio de Janeiro, Brazil. 2. Dental Biomaterials and Minimally Invasive Dentistry, Department of Dentistry, Cardenal Herrera-CEU University, CEU Universities, Valencia, Spain. 3. Oral Microbiology and Biomaterials Laboratory, Department of Biomedical, Surgical and Dental Sciences, Università Degli Studi Di Milano, Milan, Italy. 4. Microbiology, Departamento de Farmacia, Cardenal Herrera-CEU University, CEU Universities, Valencia, Spain. 5. Department of Biomedical Sciences for Health, Università Degli Studi Di Milano, Milan, Italy. 6. Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University UNESP, Araraquara, Brazil. 7. Dental Biomaterials and Minimally Invasive Dentistry, Department of Dentistry, Cardenal Herrera-CEU University, CEU Universities, Valencia, Spain. salvatore.sauro@uch.ceu.es.
Abstract
OBJECTIVES: Evaluate the ability of current ion-releasing materials to remineralise bacteria-driven artificial caries lesions. MATERIALS AND METHODS: Standardised class I cavities were obtained in 60 extracted human molars. Specimens underwent a microbiological cariogenic protocol (28 days) to generate artificial caries lesions and then were randomly divided into four restorative groups: adhesive + composite (negative control); glass ionomer cement (GIC); calcium silicate cement (MTA); and resin-modified calcium silicate cement (RMTA). Microhardness analysis (ΔKHN) was performed on 40 specimens (10/group, t = 30 days, 45 days, 60 days in artificial saliva, AS). Micro-CT scans were acquired (3/group, t = 0 days, 30 days, and 90 days in AS). Confocal microscopy was employed for interfacial ultra-morphology analysis (2/group, t = 0 days and 60 days in AS). Additional specimens were prepared and processed for scanning electron microscopy (SEM) and FTIR (n = 3/group + control) to analyse the ability of the tested materials to induce apatite formation on totally demineralised dentine discs (60 days in AS). Statistical analyses were performed with a significance level of 5%. RESULTS: Adhesive + composite specimens showed the lowest ΔKHN values and the presence of gaps at the interface when assessed through micro-CT even after storage in AS. Conversely, all the tested ion-releasing materials presented an increase in ΔKHN after storage (p < 0.05), while MTA best reduced the demineralised artificial carious lesions gap at the interface. MTA and RMTA also showed apatite deposition on totally demineralised dentine surfaces (SEM and FTIR). CONCLUSIONS: All tested ion-releasing materials expressed mineral precipitation in demineralised dentine. Additionally, calcium silicate-based materials induced apatite precipitation and hardness recovery of artificial carious dentine lesions over time. CLINICAL RELEVANCE: Current ion-releasing materials can induce remineralisation of carious dentine. MTA shows enhanced ability of nucleation/precipitation of hydroxyapatite compared to RMTA and GIC, which may be more appropriate to recover severe mineral-depleted dentine.
OBJECTIVES: Evaluate the ability of current ion-releasing materials to remineralise bacteria-driven artificial caries lesions. MATERIALS AND METHODS: Standardised class I cavities were obtained in 60 extracted human molars. Specimens underwent a microbiological cariogenic protocol (28 days) to generate artificial caries lesions and then were randomly divided into four restorative groups: adhesive + composite (negative control); glass ionomer cement (GIC); calcium silicate cement (MTA); and resin-modified calcium silicate cement (RMTA). Microhardness analysis (ΔKHN) was performed on 40 specimens (10/group, t = 30 days, 45 days, 60 days in artificial saliva, AS). Micro-CT scans were acquired (3/group, t = 0 days, 30 days, and 90 days in AS). Confocal microscopy was employed for interfacial ultra-morphology analysis (2/group, t = 0 days and 60 days in AS). Additional specimens were prepared and processed for scanning electron microscopy (SEM) and FTIR (n = 3/group + control) to analyse the ability of the tested materials to induce apatite formation on totally demineralised dentine discs (60 days in AS). Statistical analyses were performed with a significance level of 5%. RESULTS: Adhesive + composite specimens showed the lowest ΔKHN values and the presence of gaps at the interface when assessed through micro-CT even after storage in AS. Conversely, all the tested ion-releasing materials presented an increase in ΔKHN after storage (p < 0.05), while MTA best reduced the demineralised artificial carious lesions gap at the interface. MTA and RMTA also showed apatite deposition on totally demineralised dentine surfaces (SEM and FTIR). CONCLUSIONS: All tested ion-releasing materials expressed mineral precipitation in demineralised dentine. Additionally, calcium silicate-based materials induced apatite precipitation and hardness recovery of artificial carious dentine lesions over time. CLINICAL RELEVANCE: Current ion-releasing materials can induce remineralisation of carious dentine. MTA shows enhanced ability of nucleation/precipitation of hydroxyapatite compared to RMTA and GIC, which may be more appropriate to recover severe mineral-depleted dentine.
Authors: Sebastian Hahnel; Andrei C Ionescu; Gloria Cazzaniga; Marco Ottobelli; Eugenio Brambilla Journal: J Dent Date: 2017-02-14 Impact factor: 4.379
Authors: A Almahdy; F C Downey; S Sauro; R J Cook; M Sherriff; D Richards; T F Watson; A Banerjee; F Festy Journal: Caries Res Date: 2012-06-27 Impact factor: 4.056
Authors: F Schwendicke; J E Frencken; L Bjørndal; M Maltz; D J Manton; D Ricketts; K Van Landuyt; A Banerjee; G Campus; S Doméjean; M Fontana; S Leal; E Lo; V Machiulskiene; A Schulte; C Splieth; A F Zandona; N P T Innes Journal: Adv Dent Res Date: 2016-05