A A Alhasyimi1, P P Pudyani1, W Asmara2, I D Ana3. 1. Department of Orthodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia. 2. Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia. 3. Department of Dental Biomedical Sciences, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Abstract
OBJECTIVE: To evaluate the effect of carbonated hydroxyapatite-incorporated advanced platelet-rich fibrin on relapse and bone remodelling in rabbits. MATERIALS AND METHODS: Forty-five rabbits were divided into 3 groups (n = 15): a control group, carbonated hydroxyapatite (CHA) and carbonated hydroxyapatite-incorporated advanced platelet-rich fibrin (CHA-aPRF) group. The lower incisors were subjected to an orthodontic force of 50 cN. During the retention period, CHA and CHA-aPRF hydrogel were gently injected in the mesial gingival sulcus every 7 days. Next, the appliances were debonded to allow relapse. At 5 subsequent time points (0, 3, 7, 14 and 21 days after debonding), the distance between the mesial tips of rabbits incisors was measured using a digital calliper, osteoblast quantity was evaluated using haematoxylin-eosin staining and osteoclast activity was examined using tartrate-resistant acid phosphatase (TRAP) staining. RESULTS: The relapse rate of CHA-aPRF group was lower than that of the other groups, and the relapse distances were significantly lower on days 14 and 21 (P < .05). Histological analysis revealed that the number of osteoblasts was significantly higher and that the osteoclast activity was significantly lower in the CHA-aPRF group compared with the other groups (P < .05). CONCLUSIONS: Intrasulcular injection of hydrogel CHA incorporated aPRF can locally reduce orthodontic relapse in rabbits.
OBJECTIVE: To evaluate the effect of carbonated hydroxyapatite-incorporated advanced platelet-rich fibrin on relapse and bone remodelling in rabbits. MATERIALS AND METHODS: Forty-five rabbits were divided into 3 groups (n = 15): a control group, carbonated hydroxyapatite (CHA) and carbonated hydroxyapatite-incorporated advanced platelet-rich fibrin (CHA-aPRF) group. The lower incisors were subjected to an orthodontic force of 50 cN. During the retention period, CHA and CHA-aPRF hydrogel were gently injected in the mesial gingival sulcus every 7 days. Next, the appliances were debonded to allow relapse. At 5 subsequent time points (0, 3, 7, 14 and 21 days after debonding), the distance between the mesial tips of rabbits incisors was measured using a digital calliper, osteoblast quantity was evaluated using haematoxylin-eosin staining and osteoclast activity was examined using tartrate-resistant acid phosphatase (TRAP) staining. RESULTS: The relapse rate of CHA-aPRF group was lower than that of the other groups, and the relapse distances were significantly lower on days 14 and 21 (P < .05). Histological analysis revealed that the number of osteoblasts was significantly higher and that the osteoclast activity was significantly lower in the CHA-aPRF group compared with the other groups (P < .05). CONCLUSIONS: Intrasulcular injection of hydrogel CHA incorporated aPRF can locally reduce orthodontic relapse in rabbits.
Authors: Sebastian Blatt; Daniel G E Thiem; Solomiya Kyyak; Andreas Pabst; Bilal Al-Nawas; Peer W Kämmerer Journal: Front Bioeng Biotechnol Date: 2021-03-16