Vacis Tatarunas1, Algirdas Voleisis2, Reimondas Sliteris2, Rymantas Kazys2, Liudas Mazeika2, Vaiva Lesauskaite3. 1. Laboratory of Molecular Cardiology, Institute of Cardiology, Lithuanian University of Health Sciences, Sukileliu 15, 50009, Kaunas, Lithuania. vacis.tatarunas@lsmuni.lt. 2. Ultrasound Institute, Kaunas University of Technology, K. Baršausko g. 59, Kaunas, Lithuania. 3. Laboratory of Molecular Cardiology, Institute of Cardiology, Lithuanian University of Health Sciences, Sukileliu 15, 50009, Kaunas, Lithuania.
Abstract
PURPOSE: For long time, blood clot retraction was measured only by thromboelastographic or platelet contractile force measurement techniques. The purpose of the present study was development of a novel ultrasonic method based on simultaneous monitoring of variations in the ultrasound velocity and the frequency spectrum of the signal propagating in clotting blood and its application for automatic evaluation of blood clotting parameters. METHODS: Simultaneous measurement of ultrasound velocity and variations in the frequency spectrum of wideband ultrasonic signals in clotting blood samples was performed. All measurements were performed in pulse-echo mode. Standard clinical data were obtained using routine clinical laboratory methods. RESULTS: The amplitudes of ultrasonic signals during native blood coagulation varied up to ten times for different frequencies. The measurement results of the start and duration of blood clot retraction differed between patient samples: different components of the blood coagulation system had significant impact on the blood clot retraction process. CONCLUSIONS: Our results showed that during blood clotting, the ultrasound velocity and variations in frequency spectrum should be used simultaneously to determine the beginning and duration of blood clot retraction. Our results also showed that blood clot retraction is controlled by the activity of factor XIII.
PURPOSE: For long time, blood clot retraction was measured only by thromboelastographic or platelet contractile force measurement techniques. The purpose of the present study was development of a novel ultrasonic method based on simultaneous monitoring of variations in the ultrasound velocity and the frequency spectrum of the signal propagating in clotting blood and its application for automatic evaluation of blood clotting parameters. METHODS: Simultaneous measurement of ultrasound velocity and variations in the frequency spectrum of wideband ultrasonic signals in clotting blood samples was performed. All measurements were performed in pulse-echo mode. Standard clinical data were obtained using routine clinical laboratory methods. RESULTS: The amplitudes of ultrasonic signals during native blood coagulation varied up to ten times for different frequencies. The measurement results of the start and duration of blood clot retraction differed between patient samples: different components of the blood coagulation system had significant impact on the blood clot retraction process. CONCLUSIONS: Our results showed that during blood clotting, the ultrasound velocity and variations in frequency spectrum should be used simultaneously to determine the beginning and duration of blood clot retraction. Our results also showed that blood clot retraction is controlled by the activity of factor XIII.
Authors: Janine E van Loon; Moniek P M de Maat; Jaap W Deckers; Ron T van Domburg; Frank W G Leebeek Journal: Atherosclerosis Date: 2012-07-07 Impact factor: 5.162
Authors: Anna M Kucharska-Newton; Keri L Monda; Stephen Campbell; Patrick T Bradshaw; Lynne E Wagenknecht; Eric Boerwinkle; Bruce A Wasserman; Gerardo Heiss Journal: Atherosclerosis Date: 2011-02-02 Impact factor: 5.162