Literature DB >> 29530852

Constitutive Activation of MexT by Amino Acid Substitutions Results in MexEF-OprN Overproduction in Clinical Isolates of Pseudomonas aeruginosa.

Paulo Juarez1,2, Isabelle Broutin3, Christophe Bordi4, Patrick Plésiat5,6, Catherine Llanes1.   

Abstract

When overproduced, the multidrug efflux system MexEF-OprN increases the resistance of Pseudomonas aeruginosa to fluoroquinolones, chloramphenicol, and trimethoprim. In this work, we demonstrate that gain-of-function mutations in the regulatory gene mexT result in oligomerization of the LysR regulator MexT, constitutive upregulation of the efflux pump, and increased resistance in clinical isolates.
Copyright © 2018 American Society for Microbiology.

Entities:  

Keywords:  MexEF-OprN; MexT; Pseudomonas aeruginosa; antibiotic resistance

Mesh：

Substances：

Year:  2018        PMID： 29530852      PMCID： PMC5923100          DOI： 10.1128/AAC.02445-17

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  18 in total

1.  Characterization of MexT, the regulator of the MexE-MexF-OprN multidrug efflux system of Pseudomonas aeruginosa.

Authors:  T Köhler; S F Epp; L K Curty; J C Pechère
Journal:  J Bacteriol       Date:  1999-10       Impact factor: 3.490

2.  Amino Acid Substitutions Account for Most MexS Alterations in Clinical nfxC Mutants of Pseudomonas aeruginosa.

Authors:  Charlotte Richardot; Paulo Juarez; Katy Jeannot; Isabelle Patry; Patrick Plésiat; Catherine Llanes
Journal:  Antimicrob Agents Chemother       Date:  2016-03-25       Impact factor: 5.191

Review 3.  Structure and function of the LysR-type transcriptional regulator (LTTR) family proteins.

Authors:  Sarah E Maddocks; Petra C F Oyston
Journal:  Microbiology       Date:  2008-12       Impact factor: 2.777

4.  Inducer responses of BenM, a LysR-type transcriptional regulator from Acinetobacter baylyi ADP1.

Authors:  Sarah H Craven; Obidimma C Ezezika; Sandra Haddad; Ruth A Hall; Cory Momany; Ellen L Neidle
Journal:  Mol Microbiol       Date:  2009-04-08       Impact factor: 3.501

5.  Characterization of MexE-MexF-OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosa.

Authors:  T Köhler; M Michéa-Hamzehpour; U Henze; N Gotoh; L K Curty; J C Pechère
Journal:  Mol Microbiol       Date:  1997-01       Impact factor: 3.501

6.  Crystal structures of DntR inducer binding domains in complex with salicylate offer insights into the activation of LysR-type transcriptional regulators.

Authors:  Laurence Devesse; Irina Smirnova; Rosa Lönneborg; Ulrike Kapp; Peter Brzezinski; Gordon A Leonard; Cyril Dian
Journal:  Mol Microbiol       Date:  2011-06-22       Impact factor: 3.501

7.  Diversity of β-lactam resistance mechanisms in cystic fibrosis isolates of Pseudomonas aeruginosa: a French multicentre study.

Authors:  Catherine Llanes; Christine Pourcel; Charlotte Richardot; Patrick Plésiat; Gwennaele Fichant; Jean-Didier Cavallo; Audrey Mérens
Journal:  J Antimicrob Chemother       Date:  2013-04-29       Impact factor: 5.790

8.  MexT functions as a redox-responsive regulator modulating disulfide stress resistance in Pseudomonas aeruginosa.

Authors:  Emilie Fargier; Micheál Mac Aogáin; Marlies J Mooij; David F Woods; John P Morrissey; Alan D W Dobson; Claire Adams; Fergal O'Gara
Journal:  J Bacteriol       Date:  2012-04-27       Impact factor: 3.490

9.  Genetic markers of widespread extensively drug-resistant Pseudomonas aeruginosa high-risk clones.

Authors:  Gabriel Cabot; Alain A Ocampo-Sosa; M Angeles Domínguez; Juan F Gago; Carlos Juan; Fe Tubau; Cristina Rodríguez; Bartolomé Moyà; Carmen Peña; Luis Martínez-Martínez; Antonio Oliver
Journal:  Antimicrob Agents Chemother       Date:  2012-10-08       Impact factor: 5.191

10.  Enhancement of the mexAB-oprM efflux pump expression by a quorum-sensing autoinducer and its cancellation by a regulator, MexT, of the mexEF-oprN efflux pump operon in Pseudomonas aeruginosa.

Authors:  Hideaki Maseda; Isao Sawada; Kohjiro Saito; Hiroo Uchiyama; Taiji Nakae; Nobuhiko Nomura
Journal:  Antimicrob Agents Chemother       Date:  2004-04       Impact factor: 5.191
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  10 in total

1.  Pseudomonas aeruginosa mexT is an indicator of PAO1 strain integrity.

Authors:  Eric D LoVullo; Herbert P Schweizer
Journal:  J Med Microbiol       Date:  2019-12-18       Impact factor: 2.472

2.  Loss of RNA Chaperone Hfq Unveils a Toxic Pathway in Pseudomonas aeruginosa.

Authors:  Ian T Hill; Thomas Tallo; Matthew J Dorman; Simon L Dove
Journal:  J Bacteriol       Date:  2019-09-20       Impact factor: 3.490

Review 3.  Resistance Is Not Futile: The Role of Quorum Sensing Plasticity in Pseudomonas aeruginosa Infections and Its Link to Intrinsic Mechanisms of Antibiotic Resistance.

Authors:  Kayla A Simanek; Jon E Paczkowski
Journal:  Microorganisms       Date:  2022-06-18

Review 4.  Functional Mechanism of the Efflux Pumps Transcription Regulators From Pseudomonas aeruginosa Based on 3D Structures.

Authors:  Karim Housseini B Issa; Gilles Phan; Isabelle Broutin
Journal:  Front Mol Biosci       Date:  2018-06-19

5.  Crystal Structure of the Regulatory Domain of MexT, a Transcriptional Activator of the MexEFOprN Efflux Pump in Pseudomonas aeruginosa.

Authors:  Suhyeon Kim; Songhee H Kim; Jinsook Ahn; Inseong Jo; Zee-Won Lee; Sang Ho Choi; NamChul Ha
Journal:  Mol Cells       Date:  2019-12-31       Impact factor: 5.034

6.  13-(2-Methylbenzyl) Berberine Is a More Potent Inhibitor of MexXY-Dependent Aminoglycoside Resistance than Berberine.

Authors:  Kenta Kotani; Mio Matsumura; Yuji Morita; Junko Tomida; Ryo Kutsuna; Kunihiko Nishino; Shuji Yasuike; Yoshiaki Kawamura
Journal:  Antibiotics (Basel)       Date:  2019-11-06

7.  Microevolution of the mexT and lasR Reinforces the Bias of Quorum Sensing System in Laboratory Strains of Pseudomonas aeruginosa PAO1.

Authors:  Yang Liu; Stephen Dela Ahator; Huishan Wang; Qishun Feng; Yinuo Xu; Chuhao Li; Xiaofan Zhou; Lian-Hui Zhang
Journal:  Front Microbiol       Date:  2022-04-12       Impact factor: 6.064

8.  Efficacy of Antibiotic Combinations against Multidrug-Resistant Pseudomonas aeruginosa in Automated Time-Lapse Microscopy and Static Time-Kill Experiments.

Authors:  Anna Olsson; Pikkei Wistrand-Yuen; Elisabet I Nielsen; Lena E Friberg; Linus Sandegren; Pernilla Lagerbäck; Thomas Tängdén
Journal:  Antimicrob Agents Chemother       Date:  2020-05-21       Impact factor: 5.191

9.  Predicting antimicrobial resistance in Pseudomonas aeruginosa with machine learning-enabled molecular diagnostics.

Authors:  Ariane Khaledi; Aaron Weimann; Monika Schniederjans; Ehsaneddin Asgari; Tzu-Hao Kuo; Antonio Oliver; Gabriel Cabot; Axel Kola; Petra Gastmeier; Michael Hogardt; Daniel Jonas; Mohammad Rk Mofrad; Andreas Bremges; Alice C McHardy; Susanne Häussler
Journal:  EMBO Mol Med       Date:  2020-02-12       Impact factor: 12.137

10.  Triclosan depletes the membrane potential in Pseudomonas aeruginosa biofilms inhibiting aminoglycoside induced adaptive resistance.

Authors:  Michael M Maiden; Christopher M Waters
Journal:  PLoS Pathog       Date:  2020-10-30       Impact factor: 6.823
  10 in total

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