Thamires Priscila Cavazana1, Juliano Pelim Pessan1, Thayse Yumi Hosida1, Douglas Roberto Monteiro2, Alberto Carlos Botazzo Delbem3. 1. São Paulo State University (Unesp), School of Dentistry, Department of Pediatric Dentistry and Public Health, 16015-050, Araçatuba, São Paulo, Brazil. 2. Graduate Program in Dentistry (GPD - Master's Degree), University of Western São Paulo (UNOESTE), 19050-920, Presidente Prudente, São Paulo, Brazil. 3. São Paulo State University (Unesp), School of Dentistry, Department of Pediatric Dentistry and Public Health, 16015-050, Araçatuba, São Paulo, Brazil. Electronic address: adelbem@foa.unesp.br.
Abstract
OBJECTIVE: This study aimed to standardize an in vitro experimental model able to reproduce the pH changes that occur in dental biofilm under in vivo conditions, using a mixed biofilm of Streptococcus mutans and Candida albicans. DESIGN: Biofilms were developed for 96 h, and exposed to three different concentrations of sucrose (10, 20 or 30%) during 1, 3 or 5 min. The pH was measured before exposure to sucrose, immediately after its removal from the biofilms, and at 1, 3, 5 and 10 min after removal. RESULTS: Sucrose solutions at 10 and 20% required 1 min to significantly reduce the biofilm pH, while for 30% sucrose a significant reduction was already seen immediately after its removal, even for the shortest exposure time. For an exposure of 3 min to 20% sucrose, the biofilm pH attained the critical value for hydroxyapatite dissolution when measured 1 min after sucrose removal, followed by a recovery phase. CONCLUSIONS: A mixed biofilm of S. mutans and C. albicans exposed to a 20% sucrose solution for 3 min exhibited a pattern of pH change similar to that observed in vivo, despite at a higher speed when compared to in vivo conditions.
OBJECTIVE: This study aimed to standardize an in vitro experimental model able to reproduce the pH changes that occur in dental biofilm under in vivo conditions, using a mixed biofilm of Streptococcus mutans and Candida albicans. DESIGN: Biofilms were developed for 96 h, and exposed to three different concentrations of sucrose (10, 20 or 30%) during 1, 3 or 5 min. The pH was measured before exposure to sucrose, immediately after its removal from the biofilms, and at 1, 3, 5 and 10 min after removal. RESULTS:Sucrose solutions at 10 and 20% required 1 min to significantly reduce the biofilm pH, while for 30% sucrose a significant reduction was already seen immediately after its removal, even for the shortest exposure time. For an exposure of 3 min to 20% sucrose, the biofilm pH attained the critical value for hydroxyapatite dissolution when measured 1 min after sucrose removal, followed by a recovery phase. CONCLUSIONS: A mixed biofilm of S. mutans and C. albicans exposed to a 20% sucrose solution for 3 min exhibited a pattern of pH change similar to that observed in vivo, despite at a higher speed when compared to in vivo conditions.
Authors: Francesca Di Cristo; Anna Valentino; Ilenia De Luca; Gianfranco Peluso; Irene Bonadies; Anna Calarco; Anna Di Salle Journal: Molecules Date: 2022-03-28 Impact factor: 4.411