Sahar Ghanmi1, Moez Trigui2, Walid Baya2, Zoubaier Ellouz2, Abdelfatteh Elfeki3, Slim Charfi4, Jean Christophe Fricain5, Hassib Keskes2. 1. Experimental Surgery of the Musculoskeletal System Laboratory, Sfax Faculty of Medicine, Sfax, Tunisia; Tissue Bioengineering Laboratory, U1026, Inserm, University of Bordeaux, France. Electronic address: ghanmisahar@yahoo.fr. 2. Experimental Surgery of the Musculoskeletal System Laboratory, Sfax Faculty of Medicine, Sfax, Tunisia. 3. Animal Ecophysiology Laboratory, Sfax Faculty of Science, Department of Life Sciences, Sfax, Tunisia. 4. Anatomy and Pathology Services, Hospital Habib Bourgiba, Sfax, Tunisia. 5. Tissue Bioengineering Laboratory, U1026, Inserm, University of Bordeaux, France.
Abstract
OBJECTIVE: The purpose of this study was to investigate the effect of fresh human amniotic membrane (FHAM) as a substitute of periosteum to enhance bone regeneration in critical-sized defects. METHODS: Tibial diaphyseal bone defects were created in forty New Zealand white rabbits and treated with FHAM or left empty. Treatment groups consisted of: FHAM implanted in the place of removed periosteum (FHAMP group); FHFAM implanted to fill the entire defect (FHAMF group) compared to negative control group; empty defect with removing the periosteum (NC group) and positive control group; and empty defect without removing the periosteum (PC group). Bone regeneration was evaluated by radiographic, micro-computed tomography (μ-CT) and histological analyses at 4 and 8weeks post-surgery. RESULTS: Radiographic and μ-CT analysis demonstrated clearly enhanced new bone formation in positive control group (PC) and FHAMP group compared to negative control group (NC) and FHAMF group. Histological staining exhibited remaining woven bones and cartilage matrix in the FHAMP group, immature lamellar bone with medellury cavity and marrow bone formation in PC group from 4weeks post-operatively. For FHAMF group, a little new bone formation was detected only from 8weeks post-operatively, and an absence of any sign of healing in NC group at both time points. CONCLUSION: The results provide that FHAM increases bone regeneration in critical-sized defects when it is implanted in the place of the removed periosteum, but its additive effect does not have the same effect of the natural periosteum.
OBJECTIVE: The purpose of this study was to investigate the effect of fresh human amniotic membrane (FHAM) as a substitute of periosteum to enhance bone regeneration in critical-sized defects. METHODS: Tibial diaphyseal bone defects were created in forty New Zealand white rabbits and treated with FHAM or left empty. Treatment groups consisted of: FHAM implanted in the place of removed periosteum (FHAMP group); FHFAM implanted to fill the entire defect (FHAMF group) compared to negative control group; empty defect with removing the periosteum (NC group) and positive control group; and empty defect without removing the periosteum (PC group). Bone regeneration was evaluated by radiographic, micro-computed tomography (μ-CT) and histological analyses at 4 and 8weeks post-surgery. RESULTS: Radiographic and μ-CT analysis demonstrated clearly enhanced new bone formation in positive control group (PC) and FHAMP group compared to negative control group (NC) and FHAMF group. Histological staining exhibited remaining woven bones and cartilage matrix in the FHAMP group, immature lamellar bone with medellury cavity and marrow bone formation in PC group from 4weeks post-operatively. For FHAMF group, a little new bone formation was detected only from 8weeks post-operatively, and an absence of any sign of healing in NC group at both time points. CONCLUSION: The results provide that FHAM increases bone regeneration in critical-sized defects when it is implanted in the place of the removed periosteum, but its additive effect does not have the same effect of the natural periosteum.