| Literature DB >> 29517505 |
Achim A Jungbluth1, Denise Frosina1, Miriam Fayad1, Melissa P Pulitzer1, Ahmet Dogan1, Klaus J Busam1, Naoko Imai2, Sacha Gnjatic2.
Abstract
T lymphocytes can be distinguished based on the composition of the T-cell receptor (TCR) chain in α/β T cells and γ/δ T cells. Correspondingly, α/β lymphomas can be distinguished from γ/δ lymphomas. The latter are rare neoplasms, which are usually confined to particular organs and tissues and carry a dismal prognosis. Until recently, monoclonal antibody (mAb) clone g3.20 to the TCR γ-chain was the reagent of choice for the immunohistochemical detection of γ/δ T cells and lymphomas in standard formalin-fixed paraffin-embedded tissues. However, due to technical problems, mAb g3.20 became recently unavailable. Our attempts to identify another commercially available clone to the TCR γ-chain were unsuccessful. However, we were able to identify a mAb (clone H-41, SC-100289; Santa Cruz, Dallas, TX) to the TCR δ-chain. H-41 works well in immunohistochemistry on paraffin-embedded tissue and comparison with previously stained cases, shows superior immunolabeling to mAb g3.20. H-41 to the TCR δ-chain appears to be a suitable reagent for the replacement of mAb g3.20.Entities:
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Year: 2019 PMID： 29517505 PMCID： PMC7790162 DOI： 10.1097/PAI.0000000000000650
Source DB: PubMed Journal: Appl Immunohistochem Mol Morphol ISSN： 1533-4058