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Literature DB >> 2951581

In vivo cloning of DNA into multicopy cosmids by mini-Mu-cosduction.

Abstract

A general in vivo procedure for cloning Escherichia coli genes into cosmids has been developed. The method we describe here uses a deleted Mu phage (a mini-Mu) to transpose E. coli genes into cosmids during mini-Mu replication. The resulting cosmids clones are packaged in vivo into lambda phage particles. Plasmids carrying a particular DNA sequence can be selectively recovered after infection of a new host with the in vivo constructed genomic cosmid library. This system was used successfully to clone several E. coli genes.

Entities: Species

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Year: 1986 PMID： 2951581 DOI： 10.1007/bf00338096

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

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References

16 in total

1. Mini-muduction: a new mode of gene transfer mediated by mini-mu.

Authors: M Faelen; A Toussaint; A Resibois
Journal: Mol Gen Genet Date: 1979-10-03

2. Cloning of mini-mu bacteriophage in cosmids: in vivo packaging into phage lambda heads.

Authors: D de Mendoza; A L Rosa
Journal: Gene Date: 1985 Impact factor: 3.688

3. Mutants of Escherichia coli defective in membrane phospholipid synthesis: mapping of the structural gene for L-glycerol 3-phosphate dehydrogenase.

Authors: J E Cronan; R M Bell
Journal: J Bacteriol Date: 1974-05 Impact factor: 3.490

In vivo cloning of DNA into multicopy cosmids by mini-Mu-cosduction.

1. Mini-muduction: a new mode of gene transfer mediated by mini-mu.

2. Cloning of mini-mu bacteriophage in cosmids: in vivo packaging into phage lambda heads.

3. Mutants of Escherichia coli defective in membrane phospholipid synthesis: mapping of the structural gene for L-glycerol 3-phosphate dehydrogenase.

4. Cosmid DNA packaging in vivo.

5. Genetic study of Mu transposition and Mu-mediated chromosomal rearrangements.

6. A rapid alkaline extraction method for the isolation of plasmid DNA.

7. In vivo DNA cloning and adjacent gene fusing with a mini-Mu-lac bacteriophage containing a plasmid replicon.

8. Plasmid insertion mutagenesis and lac gene fusion with mini-mu bacteriophage transposons.

9. In vivo cloning of Erwinia carotovora genes involved in the catabolism of hexuronates.

10. A new high-capacity cosmid vector and its use.