David W Rowe1, Douglas J Adams2, Seung-Hyun Hong3, Caibin Zhang4, Dong-Guk Shin3, C Renata Rydzik2, Li Chen4, Zhihua Wu4, Gaven Garland5, Dana A Godfrey6, John P Sundberg5, Cheryl Ackert-Bicknell6. 1. Regenerative Medicine and Skeletal Development, Department of Reconstructive Sciences, Biomaterials and Skeletal Development, School of Dental Medicine, University of Connecticut Health, Farmington, CT, 06030, USA. drowe@uchc.edu. 2. Department of Orthopaedic Surgery, School of Medicine, University of Connecticut Health, Farmington, CT, 06030, USA. 3. Computer Science and Engineering, School of Engineering, University of Connecticut, Storrs, CT, 06269, USA. 4. Regenerative Medicine and Skeletal Development, Department of Reconstructive Sciences, Biomaterials and Skeletal Development, School of Dental Medicine, University of Connecticut Health, Farmington, CT, 06030, USA. 5. The Jackson Laboratory, Bar Harbor, ME, 04609, USA. 6. Center for Musculoskeletal Research, Department of Orthopaedics and Rehabilitation, University of Rochester School of Medicine, Rochester, NY, 14642, USA.
Abstract
PURPOSE OF REVIEW: The international mouse phenotyping consortium (IMPC) is producing defined gene knockout mouse lines. Here, a phenotyping program is presented that is based on micro-computed tomography (μCT) assessment of distal femur and vertebra. Lines with significant variation undergo a computer-based bone histomorphometric analysis. RECENT FINDINGS: Of the 220 lines examined to date, approximately 15% have a significant variation (high or low) by μCT, most of which are not identified by the IMPC screen. Significant dimorphism between the sexes and bone compartments adds to the complexity of the skeletal findings. The μCT information that is posted at www.bonebase.org can group KOMP lines with similar morphological features. The histological data is presented in a graphic form that associates the cellular features with a specific anatomic group. The web portal presents a bone-centric view appropriate for the skeletal biologist/clinician to organize and understand the large number of genes that can influence skeletal health. Cataloging the relative severity of each variant is the first step towards compiling the dataset necessary to appreciate the full polygenic basis of degenerative bone disease.
PURPOSE OF REVIEW: The international mouse phenotyping consortium (IMPC) is producing defined gene knockout mouse lines. Here, a phenotyping program is presented that is based on micro-computed tomography (μCT) assessment of distal femur and vertebra. Lines with significant variation undergo a computer-based bone histomorphometric analysis. RECENT FINDINGS: Of the 220 lines examined to date, approximately 15% have a significant variation (high or low) by μCT, most of which are not identified by the IMPC screen. Significant dimorphism between the sexes and bone compartments adds to the complexity of the skeletal findings. The μCT information that is posted at www.bonebase.org can group KOMP lines with similar morphological features. The histological data is presented in a graphic form that associates the cellular features with a specific anatomic group. The web portal presents a bone-centric view appropriate for the skeletal biologist/clinician to organize and understand the large number of genes that can influence skeletal health. Cataloging the relative severity of each variant is the first step towards compiling the dataset necessary to appreciate the full polygenic basis of degenerative bone disease.
Entities:
Keywords:
Bone phenotyping; Histomorphometry; IMPC and KOMP; Web portal; μCT
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