Optimization of mixed surfactants-based β-carotene nanoemulsions using response surface methodology: An ultrasonic homogenization approach.

In the present study, food grade mixed surfactant-based β-carotene nanoemulsions were prepared without using any co-surfactant. Response surface methodology (RSM) along with central composite design (CCD) was used to investigate the effect of independent variables (surfactant concentration, ultrasonic homogenization time and oil content) on response variables. RSM analysis results revealed that experimental results were best fitted into a quadratic polynomial model with regression coefficient values of more than 0.900 for all responses. Optimized preparation conditions for β-carotene nanoemulsions were 5.82% surfactant concentration, 4 min ultrasonic homogenization time and 6.50% oil content. The experimental values at optimized preparation conditions were 119.33 nm droplet size, 2.67p-Anisidine value and 85.63% β-carotene retention. This study will be helpful for the fortification of aqueous products with β-carotene.