| Literature DB >> 29492273 |
Melissa Laird Smith1,2,3,4,5,6,7,8,9,10,11, Ben Murrell1,2,3,4,5,6,7,8,9,10,11, Kemal Eren1,2,3,4,5,6,7,8,9,10,11, Caroline Ignacio1,2,3,4,5,6,7,8,9,10,11, Elise Landais1,2,3,4,5,6,7,8,9,10,11, Steven Weaver1,2,3,4,5,6,7,8,9,10,11, Pham Phung1,2,3,4,5,6,7,8,9,10,11, Colleen Ludka1,2,3,4,5,6,7,8,9,10,11, Lance Hepler1,2,3,4,5,6,7,8,9,10,11, Gemma Caballero1,2,3,4,5,6,7,8,9,10,11, Tristan Pollner1,2,3,4,5,6,7,8,9,10,11, Yan Guo1,2,3,4,5,6,7,8,9,10,11, Douglas Richman1,2,3,4,5,6,7,8,9,10,11, Pascal Poignard1,2,3,4,5,6,7,8,9,10,11, Ellen E Paxinos1,2,3,4,5,6,7,8,9,10,11, Sergei L Kosakovsky Pond1,2,3,4,5,6,7,8,9,10,11, Davey M Smith1,2,3,4,5,6,7,8,9,10,11.
Abstract
The ability to study rapidly evolving viral populations has been constrained by the read length of next-generation sequencing approaches and the sampling depth of single-genome amplification methods. Here, we develop and characterize a method using Pacific Biosciences' Single Molecule, Real-Time (SMRT®) sequencing technology to sequence multiple, intact full-length human immunodeficiency virus-1 env genes amplified from viral RNA populations circulating in blood, and provide computational tools for analyzing and visualizing these data.Entities:
Keywords: HIV envelope; deep sequencing
Year: 2016 PMID： 29492273 PMCID： PMC5822884 DOI： 10.1093/ve/vew018
Source DB: PubMed Journal: Virus Evol ISSN： 2057-1577