Magda S S Moutinho1, Sofia Aragão2, Diogo Carmo3, Fátima Casaca3, Sandra Silva3, Joana Ribeiro4, Hugo Sousa4, Isabel Pires5,6, Felisbina Queiroga2,5,7, Bruno Colaço2,8, Rui Medeiros4,9,10,11, Paula A Oliveira2,5, Carlos Lopes3, Margarida M S M Bastos12, Rui M Gil DA Costa2,4,12. 1. Center for the Research and Technology of Agro-Environmental and Biological Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal magdamoutinho@hotmail.com. 2. Center for the Research and Technology of Agro-Environmental and Biological Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal. 3. Botelho Moniz Análises Clínicas, Porto, Portugal. 4. Molecular Oncology and Viral Pathology Group, IPO-Porto Research Center, Portuguese Institute of Oncology of Porto, Porto, Portugal. 5. Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal. 6. Animal and Veterinary Research Center-Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal. 7. Center for the Study of Animal Science, University of Porto, Porto, Portugal. 8. Zootechnics Department, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal. 9. Faculty of Medicine, University of Porto, Porto, Portugal. 10. Center for Biomedical Research, Faculty of Health Sciences, Fernando Pessoa University, Porto, Portugal. 11. Portuguese League Against Cancer, Research Department (NRNorte), Porto, Portugal. 12. Laboratory for Process Engineering Environment Biotechnology and Energy (LEPABE) Chemical Engineering Department, Faculty of Engineering, University of Porto, Porto, Portugal.
Abstract
AIM: Cyclo-oxygenase-2 (COX2) plays a prominent role in carcinogenesis. This study addresses the effects of two nutraceutical compounds on the expression of COX2 and tumor-associated inflammation in human papillomavirus type 16 (HPV16)-transgenic mice. MATERIALS AND METHODS: Six-week-old FVB/n mice were supplemented with rutin or curcumin for 24 weeks: HPV16-/- no treatment, n=12; HPV16+/- no treatment, n=13; HPV16+/- rutin, n=12; HPV16+/- curcumin, n=13. HPV16-induced skin lesions and their inflammatory infiltrates were studied histologically. COX2 expression was assessed immunohistochemically. RESULTS: Rutin reduced COX2 expression in the dermis (immunostaining score 7.83 versus 11.25 in untreated HPV16-transgenic mice) and epidermis (4.5 versus 10.0). Curcumin led to dermal and epidermal scores of 10.5 and 4.5. Both compounds reduced leukocytic infiltration, but neither prevented epidermal dysplasia. CONCLUSION: COX2 expression in HPV16-induced lesions may be modulated by nutraceuticals, reducing tumor-associated inflammation. However, this was not sufficient to block carcinogenesis, calling for additional studies focused on combination therapies. Copyright
AIM: Cyclo-oxygenase-2 (COX2) plays a prominent role in carcinogenesis. This study addresses the effects of two nutraceutical compounds on the expression of COX2 and tumor-associated inflammation in human papillomavirus type 16 (HPV16)-transgenic mice. MATERIALS AND METHODS: Six-week-old FVB/n mice were supplemented with rutin or curcumin for 24 weeks: HPV16-/- no treatment, n=12; HPV16+/- no treatment, n=13; HPV16+/- rutin, n=12; HPV16+/- curcumin, n=13. HPV16-induced skin lesions and their inflammatory infiltrates were studied histologically. COX2 expression was assessed immunohistochemically. RESULTS:Rutin reduced COX2 expression in the dermis (immunostaining score 7.83 versus 11.25 in untreated HPV16-transgenic mice) and epidermis (4.5 versus 10.0). Curcumin led to dermal and epidermal scores of 10.5 and 4.5. Both compounds reduced leukocytic infiltration, but neither prevented epidermal dysplasia. CONCLUSION: COX2 expression in HPV16-induced lesions may be modulated by nutraceuticals, reducing tumor-associated inflammation. However, this was not sufficient to block carcinogenesis, calling for additional studies focused on combination therapies. Copyright
Authors: Tiago Ferreira; Sandra Campos; Mónica G Silva; Rita Ribeiro; Susana Santos; José Almeida; Maria João Pires; Rui Miguel Gil da Costa; Cláudia Córdova; António Nogueira; Maria João Neuparth; Rui Medeiros; Margarida Maria da Silva Monteiro Bastos; Isabel Gaivão; Francisco Peixoto; Maria Manuel Oliveira; Paula Alexandra Oliveira Journal: Int J Mol Sci Date: 2019-08-10 Impact factor: 5.923
Authors: Pedro Rodrigues; Tiago Ferreira; Elisabete Nascimento-Gonçalves; Fernanda Seixas; Rui Miguel Gil da Costa; Tânia Martins; Maria João Neuparth; Maria João Pires; Germano Lanzarin; Luís Félix; Carlos Venâncio; Isabel C F R Ferreira; Margarida M S M Bastos; Rui Medeiros; Isabel Gaivão; Eduardo Rosa; Paula A Oliveira Journal: Biomedicines Date: 2020-04-04