Literature DB >> 29474841

Extraction of high-quality RNA from Bacillus subtilis with a lysozyme pre-treatment followed by the Trizol method.

Eber Villa-Rodríguez1, Cuauhtemoc Ibarra-Gámez1, Sergio de Los Santos-Villalobos2.   

Abstract

A suitable technique (lysozyme combined with Trizol reagent) was developed to improve the RNA yield, purity and integrity from Bacillus subtilis, under different bacterial growth stages. The obtained RNA was intact, having the required characteristics for downstream applications.
Copyright © 2018 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Bacillus subtilis; RNA Integrity Number (RIN); RNA extraction; Trizol

Mesh：

Substances：

Year:  2018        PMID： 29474841     DOI： 10.1016/j.mimet.2018.02.011

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  10 in total

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  10 in total

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