Carla Oliveira Favretto1, Alberto Carlos Botazzo Delbem1, João Carlos Silos Moraes2, Emerson Rodrigues Camargo3, Priscila Toninatto Alves de Toledo1, Denise Pedrini4,5. 1. Department of Pediatric Dentistry and Public Health, School of Dentistry, Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, Brazil. 2. Department of Physics and Chemistry, São Paulo State University (UNESP), Ilha Solteira, SP, Brazil. 3. Department of Chemistry, Federal University of São Carlos - UFSCAR, São Carlos, SP, Brazil. 4. Department of Surgery and Integrated Clinic, School of Dentistry, Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, Brazil. pedrini@foa.unesp.br. 5. Disciplina de Clínica Integrada, Faculdade de Odontologia do Campus de Araçatuba, UNESP, Rua José Bonifácio 1193, CEP, Araçatuba, SP, 16015-050, Brazil. pedrini@foa.unesp.br.
Abstract
OBJECTIVES: This in vitro study evaluated the effect of microparticles (TMPmicro) or nanoparticles (TMPnano) TMP associated with fluoride (F) in toothpaste formulations on the obliteration of dentinal tubules (DT). MATERIALS AND METHODS: The dentinal tubules of bovine dentin blocks were unobstructed by immersion in 37% phosphoric acid solution. Blocks (n = 20/group) underwent mechanical brushing (2×/day) during 7 days with toothpastes: placebo, 1100 ppm F, and 1100 with 3% TMPmicro or 3% TMPnano. After that, ten blocks of each group were immersed in citric acid (pH 3.2) for 1 min. The number, diameter and area of unobstructed DT, atomic % of chemical elements from precipitates, and the mineral concentration were quantified. Data were submitted to two-way ANOVA, followed by Student-Newman-Keuls test (P < 0.05). RESULTS: Toothpastes containing TMPmicro and TMPnano promoted greater obliteration of DT and greater mineral concentration compared to other groups (P < 0.05). Placebo and 1100 ppm F group presented similar obliteration but 1100 ppm F group promoted higher mineral concentration. Higher Ca/P ratios were observed in groups treated with TMP; the acid challenge reduced Ca/P ratio for all groups. The atomic % of C and N was significantly lower for TMP toothpastes before acid challenge, but increased afterwards. CONCLUSIONS: It was concluded that the addition of TMPmicro or TMPnano to F toothpastes produced greater obliteration of dentinal tubules and higher mineral concentration when compared with 1100 ppm F. CLINICAL RELEVANCE: By the addition of TMP, fluoride toothpastes may occlude the dentinal tubules, with potential to reduce dentin hypersensitivity.
OBJECTIVES: This in vitro study evaluated the effect of microparticles (TMPmicro) or nanoparticles (TMPnano) TMP associated with fluoride (F) in toothpaste formulations on the obliteration of dentinal tubules (DT). MATERIALS AND METHODS: The dentinal tubules of bovine dentin blocks were unobstructed by immersion in 37% phosphoric acid solution. Blocks (n = 20/group) underwent mechanical brushing (2×/day) during 7 days with toothpastes: placebo, 1100 ppm F, and 1100 with 3% TMPmicro or 3% TMPnano. After that, ten blocks of each group were immersed in citric acid (pH 3.2) for 1 min. The number, diameter and area of unobstructed DT, atomic % of chemical elements from precipitates, and the mineral concentration were quantified. Data were submitted to two-way ANOVA, followed by Student-Newman-Keuls test (P < 0.05). RESULTS: Toothpastes containing TMPmicro and TMPnano promoted greater obliteration of DT and greater mineral concentration compared to other groups (P < 0.05). Placebo and 1100 ppm F group presented similar obliteration but 1100 ppm F group promoted higher mineral concentration. Higher Ca/P ratios were observed in groups treated with TMP; the acid challenge reduced Ca/P ratio for all groups. The atomic % of C and N was significantly lower for TMP toothpastes before acid challenge, but increased afterwards. CONCLUSIONS: It was concluded that the addition of TMPmicro or TMPnano to F toothpastes produced greater obliteration of dentinal tubules and higher mineral concentration when compared with 1100 ppm F. CLINICAL RELEVANCE: By the addition of TMP, fluoride toothpastes may occlude the dentinal tubules, with potential to reduce dentin hypersensitivity.
Authors: Raymond Schipper; Arnoud Loof; Jolan de Groot; Lucien Harthoorn; Eric Dransfield; Waander van Heerde Journal: J Chromatogr B Analyt Technol Biomed Life Sci Date: 2006-10-27 Impact factor: 3.205
Authors: Z Wang; T Jiang; S Sauro; D H Pashley; M Toledano; R Osorio; S Liang; W Xing; Y Sa; Y Wang Journal: Aust Dent J Date: 2011-10-13 Impact factor: 2.291
Authors: Eliana M Takeshita; Marcelle Danelon; Luciene P Castro; Kikue T Sassaki; Alberto C B Delbem Journal: Caries Res Date: 2015-06-24 Impact factor: 4.056