Nahla E El-Ashmawy1, Naglaa F Khedr2, Hoda A El-Bahrawy3, Omnia B Hamada4. 1. Department of Biochemistry, Faculty of Pharmacy, Tanta University, Tanta, El-Gharbia, Egypt. Electronic address: nahla.elashmawi@pharm.tanta.edu.eg. 2. Department of Biochemistry, Faculty of Pharmacy, Tanta University, Tanta, El-Gharbia, Egypt. Electronic address: naglaa.khedr@pharm.tanta.edu.eg. 3. Department of Biochemistry, Faculty of Pharmacy, Tanta University, Tanta, El-Gharbia, Egypt. Electronic address: hoda.elbahrawi@pharm.tanta.edu.eg. 4. Department of Biochemistry, Faculty of Pharmacy, Tanta University, Tanta, El-Gharbia, Egypt. Electronic address: omnia.hamada@pharm.tanta.edu.eg.
Abstract
BACKGROUND: Oxidative stress and inflammation may play a key role in the pathogenesis of acute pancreatitis (AP). Lycopene, a natural carotenoid, has antioxidant scavenger capacity and inhibits inflammation in many experimental models. AIM: The study was designed to investigate whether lycopene can ameliorate l-arginine-induced pancreatitis in rats and to elucidate the underlying molecular mechanisms of these effects. METHODS: Forty-eight adult male Wistar rats were divided into: control group (vehicle, orally, 10 days), AP group (3 g/kg l-arginine, single i.p. injection, on day 10th of the experiment), lycopene group (50 mg/kg) and methylprednisolone group (30 mg/kg). Lycopene and methylprednisolone were given orally, once daily for 10 days prior to l-arginine injection. Rats were sacrificed 24 h after l-arginine injection. Inflammation/oxidative stress and pancreatic markers were assessed. Pancreatic histopathological studies were done. RESULTS: Lycopene group showed a significant reduction in tumor necrosis factor alpha (TNF-α), myeloperoxidase activity, and down-regulation of inducible nitric oxide synthase (iNOS) gene expression. Pancreatic nitric oxide concentration was reduced and pancreatic GSH was increased in lycopene group. Serum α-amylase and lipase activities were reduced by lycopene treatment. The histology of pancreas was improved in lycopene group as well as methylprednisolone group. CONCLUSION: Lycopene prior treatment proved anti-inflammatory and antioxidant effects against AP rat model via different mechanisms.
BACKGROUND: Oxidative stress and inflammation may play a key role in the pathogenesis of acute pancreatitis (AP). Lycopene, a natural carotenoid, has antioxidant scavenger capacity and inhibits inflammation in many experimental models. AIM: The study was designed to investigate whether lycopene can ameliorate l-arginine-induced pancreatitis in rats and to elucidate the underlying molecular mechanisms of these effects. METHODS: Forty-eight adult male Wistar rats were divided into: control group (vehicle, orally, 10 days), AP group (3 g/kg l-arginine, single i.p. injection, on day 10th of the experiment), lycopene group (50 mg/kg) and methylprednisolone group (30 mg/kg). Lycopene and methylprednisolone were given orally, once daily for 10 days prior to l-arginine injection. Rats were sacrificed 24 h after l-arginine injection. Inflammation/oxidative stress and pancreatic markers were assessed. Pancreatic histopathological studies were done. RESULTS:Lycopene group showed a significant reduction in tumor necrosis factor alpha (TNF-α), myeloperoxidase activity, and down-regulation of inducible nitric oxide synthase (iNOS) gene expression. Pancreaticnitric oxide concentration was reduced and pancreaticGSH was increased in lycopene group. Serum α-amylase and lipase activities were reduced by lycopene treatment. The histology of pancreas was improved in lycopene group as well as methylprednisolone group. CONCLUSION:Lycopene prior treatment proved anti-inflammatory and antioxidant effects against AP rat model via different mechanisms.
Authors: Pedro Silva-Vaz; Ana Margarida Abrantes; Miguel Castelo-Branco; António Gouveia; Maria Filomena Botelho; José Guilherme Tralhão Journal: Int J Mol Sci Date: 2020-01-04 Impact factor: 5.923