| Literature DB >> 29434353 |
Mengyun Zhang1,2, Yong Dong1,2, Fangxiao Hu1, Dan Yang1, Qianhao Zhao1, Cui Lv1, Ying Wang1,2, Chengxiang Xia1,2, Qitong Weng1,2, Xiaofei Liu1, Chen Li3, Peiqing Zhou1,2, Tongjie Wang1, Yuxian Guan1, Rongqun Guo1,2, Lijuan Liu1, Yang Geng1, Hongling Wu1, Juan Du1, Zheng Hu4, Sheng Xu5, Jiekai Chen1,2, Aibin He3, Bing Liu6, Demin Wang7,8, Yong-Guang Yang4,9, Jinyong Wang10,11.
Abstract
Deletion of master regulators of the B cell lineage reprograms B cells into T cells. Here we found that the transcription factor Hoxb5, which is expressed in uncommitted hematopoietic progenitor cells but is not present in cells committed to the B cell or T cell lineage, was able to reprogram pro-pre-B cells into functional early T cell lineage progenitors. This reprogramming started in the bone marrow and was completed in the thymus and gave rise to T lymphocytes with transcriptomes, hierarchical differentiation, tissue distribution and immunological functions that closely resembled those of their natural counterparts. Hoxb5 repressed B cell 'master genes', activated regulators of T cells and regulated crucial chromatin modifiers in pro-pre-B cells and ultimately drove the B cell fate-to-T cell fate conversion. Our results provide a de novo paradigm for the generation of functional T cells through reprogramming in vivo.Entities:
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Year: 2018 PMID： 29434353 PMCID： PMC6190911 DOI： 10.1038/s41590-018-0046-x
Source DB: PubMed Journal: Nat Immunol ISSN： 1529-2908 Impact factor: 25.606