Literature DB >> 29430484

Characterising Maturation of GFP and mCherry of Genomically Integrated Fusions in Saccharomyces cerevisiae.

Sviatlana Shashkova1,2, Adam Jm Wollman1, Stefan Hohmann2,3, Mark C Leake1.   

Abstract

Single-molecule fluorescence microscopy enables unrivaled sub-cellular quantitation of genomically encoded fusions of native proteins with fluorescent protein reporters. Fluorescent proteins must undergo in vivo maturation after expression before they become photoactive. Maturation effects must be quantified during single-molecule analysis. Here we present a method to characterise maturation of GFP and mCherry genetic protein fusions in budding yeast Saccharomyces cerevisiae.

Entities:  

Keywords:  Fluorescence; Fluorescent protein maturation; GFP; Protein fusion; Single-molecule; Yeast; mCherry

Year:  2018        PMID： 29430484      PMCID： PMC5802385          DOI： 10.21769/BioProtoc.2710

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  9 in total

1.  Tandem fluorescent protein timers for in vivo analysis of protein dynamics.

Authors:  Anton Khmelinskii; Philipp J Keller; Anna Bartosik; Matthias Meurer; Joseph D Barry; Balca R Mardin; Andreas Kaufmann; Susanne Trautmann; Malte Wachsmuth; Gislene Pereira; Wolfgang Huber; Elmar Schiebel; Michael Knop
Journal:  Nat Biotechnol       Date:  2012-06-24       Impact factor: 54.908

2.  Stoichiometry and turnover in single, functioning membrane protein complexes.

Authors:  Mark C Leake; Jennifer H Chandler; George H Wadhams; Fan Bai; Richard M Berry; Judith P Armitage
Journal:  Nature       Date:  2006-09-13       Impact factor: 49.962

3.  The physics of life: one molecule at a time.

Authors:  Mark C Leake
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2012-12-24       Impact factor: 6.237

4.  Genetic control of the cell-division cycle in yeast. I. Detection of mutants.

Authors:  L H Hartwell; J Culotti; B Reid
Journal:  Proc Natl Acad Sci U S A       Date:  1970-06       Impact factor: 11.205

5.  Millisecond single-molecule localization microscopy combined with convolution analysis and automated image segmentation to determine protein concentrations in complexly structured, functional cells, one cell at a time.

Authors:  Adam J M Wollman; Mark C Leake
Journal:  Faraday Discuss       Date:  2015-09-30       Impact factor: 4.008

6.  In vivo architecture and action of bacterial structural maintenance of chromosome proteins.

Authors:  Anjana Badrinarayanan; Rodrigo Reyes-Lamothe; Stephan Uphoff; Mark C Leake; David J Sherratt
Journal:  Science       Date:  2012-10-26       Impact factor: 47.728

7.  Transcription factor clusters regulate genes in eukaryotic cells.

Authors:  Adam Jm Wollman; Sviatlana Shashkova; Erik G Hedlund; Rosmarie Friemann; Stefan Hohmann; Mark C Leake
Journal:  Elife       Date:  2017-08-25       Impact factor: 8.140

8.  Frequent exchange of the DNA polymerase during bacterial chromosome replication.

Authors:  Thomas R Beattie; Nitin Kapadia; Emilien Nicolas; Stephan Uphoff; Adam Jm Wollman; Mark C Leake; Rodrigo Reyes-Lamothe
Journal:  Elife       Date:  2017-03-31       Impact factor: 8.140

9.  The yeast Mig1 transcriptional repressor is dephosphorylated by glucose-dependent and -independent mechanisms.

Authors:  Sviatlana Shashkova; Adam J M Wollman; Mark C Leake; Stefan Hohmann
Journal:  FEMS Microbiol Lett       Date:  2017-08-01       Impact factor: 2.742
  9 in total
  10 in total

1.  Comparison of endogenously expressed fluorescent protein fusions behaviour for protein quality control and cellular ageing research.

Authors:  Kara L Schneider; Adam J M Wollman; Thomas Nyström; Sviatlana Shashkova
Journal:  Sci Rep       Date:  2021-06-17       Impact factor: 4.379

2.  Single-Organelle Quantification Reveals Stoichiometric and Structural Variability of Carboxysomes Dependent on the Environment.

Authors:  Yaqi Sun; Adam J M Wollman; Fang Huang; Mark C Leake; Lu-Ning Liu
Journal:  Plant Cell       Date:  2019-05-02       Impact factor: 11.277

3.  Transcription factor clusters regulate genes in eukaryotic cells.

Authors:  Adam Jm Wollman; Sviatlana Shashkova; Erik G Hedlund; Rosmarie Friemann; Stefan Hohmann; Mark C Leake
Journal:  Elife       Date:  2017-08-25       Impact factor: 8.140

4.  Single-molecule live cell imaging of Rep reveals the dynamic interplay between an accessory replicative helicase and the replisome.

Authors:  Aisha H Syeda; Adam J M Wollman; Alex L Hargreaves; Jamieson A L Howard; Jan-Gert Brüning; Peter McGlynn; Mark C Leake
Journal:  Nucleic Acids Res       Date:  2019-07-09       Impact factor: 16.971

5.  Evidence for rate-dependent filtering of global extrinsic noise by biochemical reactions in mammalian cells.

Authors:  Jiegen Wu; Xu Han; Haotian Zhai; Tingyu Yang; Yihan Lin
Journal:  Mol Syst Biol       Date:  2020-05       Impact factor: 11.429

6.  Towards mapping the 3D genome through high speed single-molecule tracking of functional transcription factors in single living cells.

Authors:  Adam J M Wollman; Erik G Hedlund; Sviatlana Shashkova; Mark C Leake
Journal:  Methods       Date:  2019-06-26       Impact factor: 3.608

7.  A glucose-starvation response governs endocytic trafficking and eisosomal retention of surface cargoes in budding yeast.

Authors:  Kamilla M E Laidlaw; Daniel D Bisinski; Sviatlana Shashkova; Katherine M Paine; Malaury A Veillon; Mark C Leake; Chris MacDonald
Journal:  J Cell Sci       Date:  2021-01-25       Impact factor: 5.285

8.  Systematic In Vivo Characterization of Fluorescent Protein Maturation in Budding Yeast.

Authors:  Paolo Guerra; Luc-Alban Vuillemenot; Brady Rae; Valeriia Ladyhina; Andreas Milias-Argeitis
Journal:  ACS Synth Biol       Date:  2022-02-18       Impact factor: 5.110

9.  Correlative single-molecule fluorescence barcoding of gene regulation in Saccharomyces cerevisiae.

Authors:  Sviatlana Shashkova; Thomas Nyström; Mark C Leake; Adam J M Wollman
Journal:  Methods       Date:  2020-10-18       Impact factor: 3.608

10.  Transcription factors in eukaryotic cells can functionally regulate gene expression by acting in oligomeric assemblies formed from an intrinsically disordered protein phase transition enabled by molecular crowding.

Authors:  Mark C Leake
Journal:  Transcription       Date:  2018-08-09
  10 in total

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