Caroline Le Duigou1, Etienne Savary2, Mélanie Morin-Brureau3, Daniel Gomez-Dominguez4, André Sobczyk3, Farah Chali3, Giampaolo Milior3, Larissa Kraus5, Jochen C Meier6, Dimitri M Kullmann7, Bertrand Mathon8, Liset Menendez de la Prida4, Georg Dorfmuller9, Johan Pallud10, Emmanuel Eugène11, Stéphane Clemenceau8, Richard Miles12. 1. Cortex & Epilepsie, Inserm U1127, CNRS UMR7225, UPMC Univ Paris 6, Institut du Cerveau et de la Moelle épinière, Paris, 75013, France, France. Electronic address: caroline.leduigou@icm-institute.org. 2. Cortex & Epilepsie, Inserm U1127, CNRS UMR7225, UPMC Univ Paris 6, Institut du Cerveau et de la Moelle épinière, Paris, 75013, France, France. Electronic address: etienne.savary@gmail.com. 3. Cortex & Epilepsie, Inserm U1127, CNRS UMR7225, UPMC Univ Paris 6, Institut du Cerveau et de la Moelle épinière, Paris, 75013, France, France. 4. Instituto Cajal, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, E-28002, Spain. 5. Cell Physiology, Technische Universität Braunschweig, Braunschweig, Germany; Charite Universitätsmedizin, Clinical and Experimental Epileptology, Berlin, Germany; Berlin Institute of Health (BIH), 10178, Berlin, Germany. 6. Cell Physiology, Technische Universität Braunschweig, Braunschweig, Germany. 7. Institute of Neurology Queens Square, University College London, UK. 8. Neurochirurgie, AP-HP, GH Pitie-Salpêtrière-Charles Foix, Paris, 75013, France. 9. Neurochirurgie, Fondation Ophtalmologique Rothschild, 75019, Paris, France. 10. Neurochirurgie, Hôpital Sainte-Anne, Paris Descartes University, IMA-BRAIN, Inserm, U894 Centre de Psychiatrie et Neurosciences, Paris, 75014, France. 11. Inserm U839, UPMC Univ Paris 6, Institut du Fer-à-Moulin, Paris, 75005, France. 12. Cortex & Epilepsie, Inserm U1127, CNRS UMR7225, UPMC Univ Paris 6, Institut du Cerveau et de la Moelle épinière, Paris, 75013, France, France. Electronic address: richard.miles@upmc.fr.
Abstract
BACKGROUND: Insights into human brain diseases may emerge from tissue obtained after operations on patients. However techniques requiring transduction of transgenes carried by viral vectors cannot be applied to acute human tissue. NEW METHOD: We show that organotypic culture techniques can be used to maintain tissue from patients with three different neurological syndromes for several weeks in vitro. Optimized viral vector techniques and promoters for transgene expression are described. RESULTS: Region-specific differences in neuronal form, firing pattern and organization as well as pathological activities were maintained over 40-50 days in culture. Both adeno-associated virus and lentivirus based vectors were persistently expressed from ∼10 days after application, providing 30-40 days to exploit genetically expressed constructs. Different promoters, including hSyn, e/hSyn, CMV and CaMKII, provided cell-type specific transgene expression. The Ca probe GCaMP let us explore epileptogenic synchrony and a FRET-based probe was used to follow activity of the kinase mTORC1. COMPARISON WITH EXISTING METHODS: The use of a defined culture medium, with low concentrations of amino acids and no growth factors, permitted organotypic culture of tissue from humans aged 3-62 years. Epileptic activity was maintained and excitability changed relatively little until ∼6 weeks in culture. CONCLUSIONS: Characteristic morphology and region-specific neuronal activities are maintained in organotypic culture of tissue from patients diagnosed with mesial temporal lobe epilepsy, cortical dysplasia and cortical glioblastoma. Viral vector techniques permit expression of probes for long-term measurements of multi-cellular activity and intra-cellular signaling.
BACKGROUND: Insights into human brain diseases may emerge from tissue obtained after operations on patients. However techniques requiring transduction of transgenes carried by viral vectors cannot be applied to acute human tissue. NEW METHOD: We show that organotypic culture techniques can be used to maintain tissue from patients with three different neurological syndromes for several weeks in vitro. Optimized viral vector techniques and promoters for transgene expression are described. RESULTS: Region-specific differences in neuronal form, firing pattern and organization as well as pathological activities were maintained over 40-50 days in culture. Both adeno-associated virus and lentivirus based vectors were persistently expressed from ∼10 days after application, providing 30-40 days to exploit genetically expressed constructs. Different promoters, including hSyn, e/hSyn, CMV and CaMKII, provided cell-type specific transgene expression. The Ca probe GCaMP let us explore epileptogenic synchrony and a FRET-based probe was used to follow activity of the kinase mTORC1. COMPARISON WITH EXISTING METHODS: The use of a defined culture medium, with low concentrations of amino acids and no growth factors, permitted organotypic culture of tissue from humans aged 3-62 years. Epileptic activity was maintained and excitability changed relatively little until ∼6 weeks in culture. CONCLUSIONS: Characteristic morphology and region-specific neuronal activities are maintained in organotypic culture of tissue from patients diagnosed with mesial temporal lobe epilepsy, cortical dysplasia and cortical glioblastoma. Viral vector techniques permit expression of probes for long-term measurements of multi-cellular activity and intra-cellular signaling.
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