Literature DB >> 29423427

Rapid IFM Dissection for Visualizing Fluorescently Tagged Sarcomeric Proteins.

Yu Shu Xiao1, Frieder Schöck1, Nicanor González-Morales1.   

Abstract

Sarcomeres, the smallest contractile unit of muscles, are arguably the most impressive actomyosin structure. Yet a complete understanding of sarcomere formation and maintenance is missing. The Drosophila indirect flight muscle (IFM) has proven to be a very valuable model to study sarcomeres. Here, we present a protocol for the rapid dissection of IFM and analysis of sarcomeres using fluorescently tagged proteins.

Entities:  

Keywords:  Dissection; Drosophila; GFP; Indirect flight muscle; Sarcomere; Z-disc

Year:  2017        PMID: 29423427      PMCID: PMC5800882          DOI: 10.21769/BioProtoc.2606

Source DB:  PubMed          Journal:  Bio Protoc        ISSN: 2331-8325


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5.  Bimolecular Fluorescence Complementation (BiFC) for Studying Sarcomeric Protein Interactions in Drosophila.

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