| Literature DB >> 29421302 |
Yi He1, Bin Wang2, Wanping Chen3, Russell J Cox4, Jingren He5, Fusheng Chen6.
Abstract
High throughput genome sequencing has revealed a multitude of potential secondary metabolites biosynthetic pathways that remain cryptic. Pathway reconstruction coupled with genetic engineering via heterologous expression enables discovery of novel compounds, elucidation of biosynthetic pathways, and optimization of product yields. Apart from Escherichia coli and yeast, fungi, especially Aspergillus spp., are well known and efficient heterologous hosts. This review summarizes recent advances in heterologous expression of microbial secondary metabolite biosynthesis in Aspergillus spp. We also discuss the technological challenges and successes in regard to heterologous host selection and DNA assembly behind the reconstruction of microbial secondary metabolite biosynthesis.Entities:
Keywords: Aspergillus spp.; Combinatorial biosynthesis; DNA assembly; Heterologous expression; Microbial secondary metabolites; Pathway engineering
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Year: 2018 PMID： 29421302 DOI： 10.1016/j.biotechadv.2018.02.001
Source DB: PubMed Journal: Biotechnol Adv ISSN： 0734-9750 Impact factor: 14.227