Literature DB >> 29411208

Stool PCR for Gastrointestinal Pathogens in Patients With and Without Immune-Mediated Intestinal Diseases.

Yael R Nobel1, Jordan Axelrad2, Suzanne K Lewis2, Susan Whittier3, Garrett Lawlor2, Simon Lichtiger2, Peter H R Green2, Benjamin Lebwohl4,5,6.   

Abstract

BACKGROUND: Patients with celiac disease and inflammatory bowel disease, two immune-mediated luminal conditions, have higher rates of certain infections than healthy counterparts. The prevalence of many gastrointestinal infections in these patients, however, is unknown. AIMS: Using a novel clinical stool pathogen PCR test, we investigated the hypothesis that patients with celiac disease/inflammatory bowel disease had different distributions of diarrheal pathogens than other patients.
METHODS: We performed a retrospective cohort study of outpatients who underwent stool pathogen testing with the FilmArray Gastrointestinal PCR Panel (BioFire Diagnostics, Salt Lake City, UT) at our institution from January 1 to December 31, 2015. Rates of pathogens were measured in patients with or without celiac disease/inflammatory bowel disease.
RESULTS: Of 955 patients, 337 had positive test for any pathogen, with 465 bacterial, parasitic, or viral pathogens identified. One hundred and twenty-seven patients (13.3%) had celiac disease or inflammatory bowel disease, of which 29/127 (22.8%) had a positive test, compared to 308/828 other patients (37.2%) (p = 0.002). Patients with celiac disease/inflammatory bowel disease had significantly fewer viruses (1.6 vs. 8.1% of patients; p = 0.008) and parasites (0 vs. 3.3%; p = 0.039), with nonsignificant trend toward fewer bacteria (21.3 vs. 29.2%; p = 0.063). Escherichia coli species were most common in both populations.
CONCLUSIONS: Stool PCR identified numerous pathogens in patients with or without celiac disease/inflammatory bowel disease. Patients with celiac disease/inflammatory bowel disease were significantly less likely to have any pathogen identified, and had significantly fewer viruses and parasites. In this population, knowledge of common pathogens can guide diagnostic evaluation and offer opportunities for treatment.

Entities:  

Keywords:  Celiac disease; Diarrhea; Enteric infections; Inflammatory bowel disease

Mesh:

Year:  2018        PMID: 29411208     DOI: 10.1007/s10620-018-4959-x

Source DB:  PubMed          Journal:  Dig Dis Sci        ISSN: 0163-2116            Impact factor:   3.199


  22 in total

1.  Risk of Clostridium difficile Infection in Patients With Celiac Disease: A Population-Based Study.

Authors:  Benjamin Lebwohl; Yael R Nobel; Peter H R Green; Martin J Blaser; Jonas F Ludvigsson
Journal:  Am J Gastroenterol       Date:  2017-10-31       Impact factor: 10.864

2.  Mucosal bacterial microflora and mucus layer thickness in adolescents with inflammatory bowel disease.

Authors:  Krzysztof Fyderek; Magdalena Strus; Kinga Kowalska-Duplaga; Tomasz Gosiewski; Andrzej Wedrychowicz; Urszula Jedynak-Wasowicz; Małgorzata Sładek; Stanisław Pieczarkowski; Paweł Adamski; Piotr Kochan; Piotr B Heczko
Journal:  World J Gastroenterol       Date:  2009-11-14       Impact factor: 5.742

3.  Multicenter evaluation of the BioFire FilmArray gastrointestinal panel for etiologic diagnosis of infectious gastroenteritis.

Authors:  Sarah N Buss; Amy Leber; Kimberle Chapin; Paul D Fey; Matthew J Bankowski; Matthew K Jones; Margarita Rogatcheva; Kristen J Kanack; Kevin M Bourzac
Journal:  J Clin Microbiol       Date:  2015-01-14       Impact factor: 5.948

Review 4.  Dysbiosis a risk factor for celiac disease.

Authors:  Anamaria Girbovan; Genel Sur; Gabriel Samasca; Iulia Lupan
Journal:  Med Microbiol Immunol       Date:  2017-02-15       Impact factor: 3.402

5.  Pneumococcal infection in patients with coeliac disease.

Authors:  Harry J Thomas; Clare J Wotton; David Yeates; Tariq Ahmad; Derek P Jewell; Michael J Goldacre
Journal:  Eur J Gastroenterol Hepatol       Date:  2008-07       Impact factor: 2.566

6.  Hepatitis B vaccine nonresponse and celiac disease.

Authors:  Kyung W Noh; Gregory A Poland; Joseph A Murray
Journal:  Am J Gastroenterol       Date:  2003-10       Impact factor: 10.864

7.  Gluten intake interferes with the humoral immune response to recombinant hepatitis B vaccine in patients with celiac disease.

Authors:  Eva Nemes; Eva Lefler; László Szegedi; Anikó Kapitány; Judit B Kovács; Márta Balogh; Katalin Szabados; Judit Tumpek; Sándor Sipka; Ilma R Korponay-Szabó
Journal:  Pediatrics       Date:  2008-06       Impact factor: 7.124

8.  Excess hospitalisation burden associated with Clostridium difficile in patients with inflammatory bowel disease.

Authors:  A N Ananthakrishnan; E L McGinley; D G Binion
Journal:  Gut       Date:  2007-09-28       Impact factor: 23.059

9.  Human leukocyte antigen-DQ2 homozygosity and the development of refractory celiac disease and enteropathy-associated T-cell lymphoma.

Authors:  Abdulbaqi Al-Toma; Marije S Goerres; Jos W R Meijer; A Salvador Peña; J Bart A Crusius; Chris J J Mulder
Journal:  Clin Gastroenterol Hepatol       Date:  2006-03       Impact factor: 11.382

10.  Failure to respond to hepatitis B vaccine in children with celiac disease.

Authors:  Seung-Dae Park; James Markowitz; Michael Pettei; Toba Weinstein; Cristina P Sison; Steven R Swiss; Jeremiah Levine
Journal:  J Pediatr Gastroenterol Nutr       Date:  2007-04       Impact factor: 2.839

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  2 in total

1.  Impact of Gastrointestinal Panel Implementation on Health Care Utilization and Outcomes.

Authors:  Jordan E Axelrad; Daniel E Freedberg; Susan Whittier; William Greendyke; Benjamin Lebwohl; Daniel A Green
Journal:  J Clin Microbiol       Date:  2019-02-27       Impact factor: 5.948

2.  Gut colonization with vancomycin-resistant Enterococcus and risk for subsequent enteric infection.

Authors:  Jordan E Axelrad; Benjamin Lebwohl; Edward Cuaresma; Ken Cadwell; Peter H R Green; Daniel E Freedberg
Journal:  Gut Pathog       Date:  2018-07-09       Impact factor: 4.181

  2 in total

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