Literature DB >> 29409823

Polymerase chain reaction (PCR) in ocular and ganglionar toxoplasmosis and the effect of therapeutics for prevention of ocular involvement in South American setting.

Jorge Enrique Gómez Marín1, Juan David Zuluaga2, Eunice Julied Pechené Campo2, Jessica Triviño2, Alejandra de-la-Torre3.   

Abstract

INTRODUCTION: Cases of toxoplasmosis present in South America tend to be more severe than that found in other continents. Here, we present our clinical experience of ocular and ganglionar toxoplasmosis in the use of PCR, and of the treatment to prevent ocular involvement.
METHODOLOGY: Retrospective analysis of clinical charts of patients with ocular and lymphadenitic toxoplasmosis at the parasitology and tropical medicine consultation in the "Universidad del Quindio" in Colombia. In total, 91 records of cases with ocular toxoplasmosis and 17 with lymphadenitis that underwent PCR analysis for B1 repeated sequence in blood, were compared to the results of 104 people with chronic asymptomatic toxoplasmosis. In addition, 41 clinical records were included from patients with confirmed toxoplasmic lymphadenitis: 10 untreated, 6 that begun treatment after four months of symptoms, and 25 that were treated during the first four months of symptoms and had a follow-up during at least one year.
RESULTS: Patients with ocular toxoplasmosis or lymphadenitis had a higher probability of PCR positivity in peripheral blood than chronic asymptomatic people. There were no cases of retinochoroiditis in 25 patients with toxoplasmic lymphadenitis treated before 4 months of symptoms and followed during at least 12 months. In four out of ten untreated cases, new lesions of retinochoroiditis presented after the symptoms of lymphadenitis.
CONCLUSIONS: Toxoplasmosisin South America exhibits different clinical behavior and this influences the laboratory results as well as the need for treatment in the case of lymphadenitis. Clinicians should be aware of the geographical origin of the infection in order to adopt different therapeutic and diagnostic approaches.
Copyright © 2018 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Colombia; Ocular toxoplasmosis; Toxoplasmic lymphadenitis; Uveitis

Mesh:

Year:  2018        PMID: 29409823     DOI: 10.1016/j.actatropica.2018.01.013

Source DB:  PubMed          Journal:  Acta Trop        ISSN: 0001-706X            Impact factor:   3.112


  5 in total

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Journal:  Biomedica       Date:  2022-03-01       Impact factor: 1.173

2.  Compressed Air-Driven Continuous-Flow Thermocycled Digital PCR for HBV Diagnosis in Clinical-Level Serum Sample Based on Single Hot Plate.

Authors:  Kangning Wang; Bin Li; Wenming Wu
Journal:  Molecules       Date:  2020-11-30       Impact factor: 4.411

3.  High frequency of ocular toxoplasmosis in Quindío, Colombia and risk factors related to the infection.

Authors:  Jorge Enrique Gómez-Marín; Juliana Muñoz-Ortiz; Manuela Mejía-Oquendo; José Y Arteaga-Rivera; Nicolás Rivera-Valdivia; María Cristina Bohórquez-Granados; Stefany Velasco-Velásquez; Gabriela Castaño-de-la-Torre; John Alejandro Acosta-Dávila; Laura Lorena García-López; Elizabeth Torres-Morales; Mónica Vargas; Juan David Valencia; Daniel Celis-Giraldo; Alejandra de-la-Torre
Journal:  Heliyon       Date:  2021-04-05

4.  Utility of blood as the clinical specimen for the diagnosis of ocular toxoplasmosis using uracil DNA glycosylase-supplemented loop-mediated isothermal amplification and real-time polymerase chain reaction assays based on REP-529 sequence and B1 gene.

Authors:  Bahman Rahimi Esboei; Shirzad Fallahi; Mohammad Zarei; Bahram Kazemi; Mehdi Mohebali; Saeedeh Shojaee; Parisa Mousavi; Aref Teimouri; Raziyeh Mahmoudzadeh; Mirataollah Salabati; Hossein Keshavarz Valian
Journal:  BMC Infect Dis       Date:  2022-01-25       Impact factor: 3.090

5.  Coinfections and differential diagnosis in immunocompetent patients with uveitis of infectious origin.

Authors:  Alejandra de-la-Torre; Juanita Valdés-Camacho; Clara López de Mesa; Andrés Uauy-Nazal; Juan David Zuluaga; Lina María Ramírez-Páez; Felipe Durán; Elizabeth Torres-Morales; Jessica Triviño; Mateo Murillo; Alba Cristina Peñaranda; Juan Carlos Sepúlveda-Arias; Jorge Enrique Gómez-Marín
Journal:  BMC Infect Dis       Date:  2019-01-25       Impact factor: 3.090

  5 in total

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