Context: Emerging evidence has related the gut microbiome and circulating metabolites to human obesity. Gut microbiota is responsible for several metabolic functions, and altered plasma metabolome might reflect differences in the gut microbiome. Objective: To identify a plasma metabolite profile associated with body mass index (BMI) in a general population and investigate whether such metabolite profile is associated with distinct composition of the gut microbiota. Design: Targeted profiling of 48 plasma metabolites was performed in a population of 920 Swedish adults (mean age, 39 years; 53% women) from the ongoing Malmö Offspring Study using targeted liquid chromatography-mass spectrometry. Gut microbiota was analyzed by sequencing the 16S ribosomal RNA gene (V1-V3 region) in fecal samples of 674 study participants. Results: BMI was associated with 19 metabolites (P < 0.001 for all), of which glutamate provided the strongest direct association (P = 5.2e-53). By orthogonal partial least squares regression, a metabolite principal component predictive of BMI was constructed (PCBMI). In addition to glutamate, PCBMI was dominated by branched-chain amino acids (BCAAs) and related metabolites. Four gut microbiota genera (Blautia, Dorea, Ruminococcus, and SHA-98) were associated with both BMI and PCBMI (P < 8.0e-4 for all). When simultaneously regressing PCBMI and metabolite-associated gut bacteria against BMI, only PCBMI remained statistically significant. Conclusions: We discovered associations between four gut microbiota genera (Blautia, Dorea, Ruminococcus, and SHA-98) and BMI-predictive plasma metabolites, including glutamate and BCAAs. Thus, these metabolites could be mediators between gut microbiota and obesity, pointing to potential future opportunities for targeting the gut microbiota in prevention of obesity.
Context: Emerging evidence has related the gut microbiome and circulating metabolites to humanobesity. Gut microbiota is responsible for several metabolic functions, and altered plasma metabolome might reflect differences in the gut microbiome. Objective: To identify a plasma metabolite profile associated with body mass index (BMI) in a general population and investigate whether such metabolite profile is associated with distinct composition of the gut microbiota. Design: Targeted profiling of 48 plasma metabolites was performed in a population of 920 Swedish adults (mean age, 39 years; 53% women) from the ongoing Malmö Offspring Study using targeted liquid chromatography-mass spectrometry. Gut microbiota was analyzed by sequencing the 16S ribosomal RNA gene (V1-V3 region) in fecal samples of 674 study participants. Results: BMI was associated with 19 metabolites (P < 0.001 for all), of which glutamate provided the strongest direct association (P = 5.2e-53). By orthogonal partial least squares regression, a metabolite principal component predictive of BMI was constructed (PCBMI). In addition to glutamate, PCBMI was dominated by branched-chain amino acids (BCAAs) and related metabolites. Four gut microbiota genera (Blautia, Dorea, Ruminococcus, and SHA-98) were associated with both BMI and PCBMI (P < 8.0e-4 for all). When simultaneously regressing PCBMI and metabolite-associated gut bacteria against BMI, only PCBMI remained statistically significant. Conclusions: We discovered associations between four gut microbiota genera (Blautia, Dorea, Ruminococcus, and SHA-98) and BMI-predictive plasma metabolites, including glutamate and BCAAs. Thus, these metabolites could be mediators between gut microbiota and obesity, pointing to potential future opportunities for targeting the gut microbiota in prevention of obesity.
Authors: Liisa Hantsoo; Eldin Jašarević; Stephanie Criniti; Brendan McGeehan; Ceylan Tanes; Mary D Sammel; Michal A Elovitz; Charlene Compher; Gary Wu; C Neill Epperson Journal: Brain Behav Immun Date: 2018-11-03 Impact factor: 7.217
Authors: Ana F Diallo; Mark B Lockwood; Katherine A Maki; Alexis T Franks; Abhrarup Roy; Rosario Jaime-Lara; Paule V Joseph; Wendy A Henderson; Seon Yoon Chung; Jacqueline McGrath; Stefan J Green; Anne M Fink Journal: Biol Res Nurs Date: 2020-07-29 Impact factor: 2.522
Authors: Louise B Thingholm; Malte C Rühlemann; Manja Koch; Brie Fuqua; Guido Laucke; Ruwen Boehm; Corinna Bang; Eric A Franzosa; Matthias Hübenthal; Ali Rahnavard; Fabian Frost; Jason Lloyd-Price; Melanie Schirmer; Aldons J Lusis; Chris D Vulpe; Markus M Lerch; Georg Homuth; Tim Kacprowski; Carsten O Schmidt; Ute Nöthlings; Tom H Karlsen; Wolfgang Lieb; Matthias Laudes; Andre Franke; Curtis Huttenhower Journal: Cell Host Microbe Date: 2019-08-06 Impact factor: 21.023
Authors: Kevin R Short; Jennifer Q Chadwick; April M Teague; Mary A Tullier; Lisa Wolbert; Charlotte Coleman; Kenneth C Copeland Journal: J Clin Endocrinol Metab Date: 2019-08-01 Impact factor: 5.958