Literature DB >> 29388135

Isolation of Extracellular RNA from Serum/Plasma.

Justyna Filant1, Parham Nejad2, Anu Paul2, Bridget Simonson3, Srimeenakshi Srinivasan4, Xuan Zhang5, Leonora Balaj5,6, Saumya Das3, Roopali Gandhi2, Louise C Laurent7, Anil K Sood1,8,9.   

Abstract

Extracellular RNAs are initiating increased interest due to their potentials in serving as novel biomarkers, mediators of intercellular communication, and therapeutic applications. As a newly emerging field, one of the main obstacles is the lack of standardized protocols for RNA isolations. Here we describe protocols for commercially available kits that have been modified to yield consistent results for isolation of extracellular RNA from both whole serum/plasma and extracellular vesicle-enriched serum/plasma samples.

Entities:  

Keywords:  Cell biology; Extracellular RNA; Extracellular Vesicle; Isolation; Molecular Biology; Nucleic acids; Purification and separation

Mesh：

Substances：

Year:  2018        PMID： 29388135      PMCID： PMC7137579          DOI： 10.1007/978-1-4939-7652-2_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  RNA-INDUCED BIOSYNTHESIS OF SPECIFIC ENZYMES.

Authors:  M C Niu; C C Cordova; L C Niu; C L Radbill
Journal:  Proc Natl Acad Sci U S A       Date:  1962-11       Impact factor: 11.205

2.  Improved method for determination of high-density-lipoprotein cholesterol I. Isolation of high-density lipoproteins by use of polyethylene glycol 6000.

Authors:  C Izzo; F Grillo; E Murador
Journal:  Clin Chem       Date:  1981-03       Impact factor: 8.327

Review 3.  Extracellular small RNAs: what, where, why?

Authors:  Anna M Hoy; Amy H Buck
Journal:  Biochem Soc Trans       Date:  2012-08       Impact factor: 5.407

4.  MicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteins.

Authors:  Kasey C Vickers; Brian T Palmisano; Bassem M Shoucri; Robert D Shamburek; Alan T Remaley
Journal:  Nat Cell Biol       Date:  2011-03-20       Impact factor: 28.824

5.  Characterization of RNA from Exosomes and Other Extracellular Vesicles Isolated by a Novel Spin Column-Based Method.

Authors:  Daniel Enderle; Alexandra Spiel; Christine M Coticchia; Emily Berghoff; Romy Mueller; Martin Schlumpberger; Markus Sprenger-Haussels; Jonathan M Shaffer; Eric Lader; Johan Skog; Mikkel Noerholm
Journal:  PLoS One       Date:  2015-08-28       Impact factor: 3.240
  5 in total
  5 in total

1.  Evaluation of a Maternal Plasma RNA Panel Predicting Spontaneous Preterm Birth and Its Expansion to the Prediction of Preeclampsia.

Authors:  Carl Philip Weiner; Howard Cuckle; Mark Louis Weiss; Irina Alexandra Buhimschi; Yafeng Dong; Helen Zhou; Risa Ramsey; Robert Egerman; Catalin Sorin Buhimschi
Journal:  Diagnostics (Basel)       Date:  2022-05-27

2.  Early pregnancy prediction of spontaneous preterm birth before 32 completed weeks of pregnancy using plasma RNA: transcriptome discovery and initial validation of an RNA panel of markers.

Authors:  C P Weiner; Y Dong; H Zhou; H Cuckle; R Ramsey; R Egerman; I Buhimschi; C Buhimschi
Journal:  BJOG       Date:  2021-05-27       Impact factor: 7.331

Review 3.  The Extracellular RNA Communication Consortium: Establishing Foundational Knowledge and Technologies for Extracellular RNA Research.

Authors:  Saumya Das; K Mark Ansel; Markus Bitzer; Xandra O Breakefield; Alain Charest; David J Galas; Mark B Gerstein; Mihir Gupta; Aleksandar Milosavljevic; Michael T McManus; Tushar Patel; Robert L Raffai; Joel Rozowsky; Matthew E Roth; Julie A Saugstad; Kendall Van Keuren-Jensen; Alissa M Weaver; Louise C Laurent
Journal:  Cell       Date:  2019-04-04       Impact factor: 66.850

4.  Asymmetric depth-filtration: A versatile and scalable method for high-yield isolation of extracellular vesicles with low contamination.

Authors:  Vasiliy S Chernyshev; Roman N Chuprov-Netochin; Ekaterina Tsydenzhapova; Elena V Svirshchevskaya; Rimma A Poltavtseva; Anastasiia Merdalimova; Alexey Yashchenok; Amiran Keshelava; Konstantin Sorokin; Varlam Keshelava; Gennadiy T Sukhikh; Dmitry Gorin; Sergey Leonov; Mikhail Skliar
Journal:  J Extracell Vesicles       Date:  2022-08

5.  Detection of Embryonic Trisomy 21 in the First Trimester Using Maternal Plasma Cell-Free RNA.

Authors:  Carl P Weiner; Mark L Weiss; Helen Zhou; Argyro Syngelaki; Kypros H Nicolaides; Yafeng Dong
Journal:  Diagnostics (Basel)       Date:  2022-06-07
  5 in total

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