Literature DB >> 29366636

Mechanism of Inhibition of Translation Termination by Blasticidin S.

Egor Svidritskiy1, Andrei A Korostelev2.   

Abstract

Understanding the mechanisms of inhibitors of translation termination may inform development of new antibacterials and therapeutics for premature termination diseases. We report the crystal structure of the potent termination inhibitor blasticidin S bound to the ribosomal 70S•release factor 1 (RF1) termination complex. Blasticidin S shifts the catalytic domain 3 of RF1 and restructures the peptidyl transferase center. Universally conserved uridine 2585 in the peptidyl transferase center occludes the catalytic backbone of the GGQ motif of RF1, explaining the structural mechanism of inhibition. Rearrangement of domain 3 relative to the codon-recognition domain 2 provides insight into the dynamics of RF1 implicated in termination accuracy.
Copyright © 2018 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  RF1; antibiotic; blasticidin S; stop-codon recognition; termination suppression

Mesh：

Substances：

Year:  2018        PMID： 29366636      PMCID： PMC5831496          DOI： 10.1016/j.jmb.2018.01.007

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  18 in total

1.  Ribosome Induces a Closed to Open Conformational Change in Release Factor 1.

Authors:  Krista Trappl; Simpson Joseph
Journal:  J Mol Biol       Date:  2016-01-29       Impact factor: 5.469

Review 2.  Structural aspects of translation termination on the ribosome.

Authors:  Andrei A Korostelev
Journal:  RNA       Date:  2011-06-23       Impact factor: 4.942

3.  Structural basis for ArfA-RF2-mediated translation termination on mRNAs lacking stop codons.

Authors:  Paul Huter; Claudia Müller; Bertrand Beckert; Stefan Arenz; Otto Berninghausen; Roland Beckmann; Daniel N Wilson
Journal:  Nature       Date:  2016-12-01       Impact factor: 49.962

4.  Structural basis of co-translational quality control by ArfA and RF2 bound to ribosome.

Authors:  Fuxing Zeng; Yanbo Chen; Jonathan Remis; Mrinal Shekhar; James C Phillips; Emad Tajkhorshid; Hong Jin
Journal:  Nature       Date:  2017-01-11       Impact factor: 49.962

5.  Blasticidin S inhibits translation by trapping deformed tRNA on the ribosome.

Authors:  Egor Svidritskiy; Clarence Ling; Dmitri N Ermolenko; Andrei A Korostelev
Journal:  Proc Natl Acad Sci U S A       Date:  2013-07-03       Impact factor: 11.205

Review 6.  Therapeutics based on stop codon readthrough.

Authors:  Kim M Keeling; Xiaojiao Xue; Gwen Gunn; David M Bedwell
Journal:  Annu Rev Genomics Hum Genet       Date:  2014-04-18       Impact factor: 8.929

7.  Crystal structure of the 70S ribosome bound with the Q253P mutant form of release factor RF2.

Authors:  Natalia Santos; Jianyu Zhu; John Paul Donohue; Andrei A Korostelev; Harry F Noller
Journal:  Structure       Date:  2013-06-13       Impact factor: 5.006

8.  Structural basis for the rescue of stalled ribosomes: structure of YaeJ bound to the ribosome.

Authors:  Matthieu G Gagnon; Sai V Seetharaman; David Bulkley; Thomas A Steitz
Journal:  Science       Date:  2012-03-16       Impact factor: 47.728

9.  An antimicrobial peptide that inhibits translation by trapping release factors on the ribosome.

Authors:  Tanja Florin; Cristina Maracci; Michael Graf; Prajwal Karki; Dorota Klepacki; Otto Berninghausen; Roland Beckmann; Nora Vázquez-Laslop; Daniel N Wilson; Marina V Rodnina; Alexander S Mankin
Journal:  Nat Struct Mol Biol       Date:  2017-07-24       Impact factor: 15.369

10.  Mechanism of ribosome rescue by ArfA and RF2.

Authors:  Gabriel Demo; Egor Svidritskiy; Rohini Madireddy; Ruben Diaz-Avalos; Timothy Grant; Nikolaus Grigorieff; Duncan Sousa; Andrei A Korostelev
Journal:  Elife       Date:  2017-03-16       Impact factor: 8.140
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  7 in total

1.  Mechanism of premature translation termination on a sense codon.

Authors:  Egor Svidritskiy; Gabriel Demo; Andrei A Korostelev
Journal:  J Biol Chem       Date:  2018-06-25       Impact factor: 5.157

2.  Conformational Control of Translation Termination on the 70S Ribosome.

Authors:  Egor Svidritskiy; Andrei A Korostelev
Journal:  Structure       Date:  2018-05-03       Impact factor: 5.006

3.  ArfB can displace mRNA to rescue stalled ribosomes.

Authors:  Christine E Carbone; Gabriel Demo; Rohini Madireddy; Egor Svidritskiy; Andrei A Korostelev
Journal:  Nat Commun       Date:  2020-11-03       Impact factor: 14.919

4.  Blasticidin S inhibits mammalian translation and enhances production of protein encoded by nonsense mRNA.

Authors:  Kyle T Powers; Flint Stevenson-Jones; Sathish K N Yadav; Beate Amthor; Joshua C Bufton; Ufuk Borucu; Dakang Shen; Jonas P Becker; Daria Lavysh; Matthias W Hentze; Andreas E Kulozik; Gabriele Neu-Yilik; Christiane Schaffitzel
Journal:  Nucleic Acids Res       Date:  2021-07-21       Impact factor: 16.971

5.  GGQ methylation enhances both speed and accuracy of stop codon recognition by bacterial class-I release factors.

Authors:  Shreya Pundir; Xueliang Ge; Suparna Sanyal
Journal:  J Biol Chem       Date:  2021-04-19       Impact factor: 5.157

Review 6.  Identification and characterization of enzymes involved in the biosynthesis of pyrimidine nucleoside antibiotics.

Authors:  M McErlean; X Liu; Z Cui; B Gust; S G Van Lanen
Journal:  Nat Prod Rep       Date:  2021-07-21       Impact factor: 15.111

7.  CTELS: A Cell-Free System for the Analysis of Translation Termination Rate.

Authors:  Kseniya A Lashkevich; Valeriya I Shlyk; Artem S Kushchenko; Vadim N Gladyshev; Elena Z Alkalaeva; Sergey E Dmitriev
Journal:  Biomolecules       Date:  2020-06-16
  7 in total

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