Hairui Luo1, Xiaohui Wang1, Yiqun Huang2, Keqiang Lai1,3, Barbara A Rasco4, Yuxia Fan1,3. 1. College of Food Science and Technology, Shanghai Ocean University, LinGang New City, Shanghai, China. 2. School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha, Hunan, China. 3. Engineering Research Center of Food Thermal Processing Technology, Shanghai Ocean University, LinGang New City, Shanghai, China. 4. School of Food Science, Washington State University, Pullman, WA, USA.
Abstract
BACKGROUND: Paraquat, a highly efficient herbicide, is widely used in agricultural practices throughout the world. However, paraquat residues in food pose a threat to human health. In order to develop a rapid and sensitive method, surface-enhanced Raman spectroscopy (SERS) coupled with gold nanoparticles was applied to analysis of paraquat in apple juice. RESULTS: Natural organic compounds (sugars and organic acids) in apple juice interfered with SERS measurement. Sample preparation was needed. Paraquat could be detected at concentrations as low as 0.02 and 0.1 µg mL- 1 with the weak cation-exchange solid-phase extraction (WCX-SPE) method and dilution method for sample preparation, respectively. For quantitative analysis, the R2 cv of the partial least-squares regression model with the dilution method (0.939) was not as good as with the WCX-SPE method (0.984), but the dilution method is much less costly, simpler and time saving. Satisfactory recovery values were obtained ranging from 94.73% to 114.81%, with the exception of 56.55% for the lowest concentration. CONCLUSION: This work showed that SERS combined with gold nanoparticles could determine paraquat in apple juice. As a simple, rapid and ultrasensitive method, it has great practical potential for detection of other contaminants in a variety of foods.
BACKGROUND:Paraquat, a highly efficient herbicide, is widely used in agricultural practices throughout the world. However, paraquat residues in food pose a threat to human health. In order to develop a rapid and sensitive method, surface-enhanced Raman spectroscopy (SERS) coupled with gold nanoparticles was applied to analysis of paraquat in apple juice. RESULTS: Natural organic compounds (sugars and organic acids) in apple juice interfered with SERS measurement. Sample preparation was needed. Paraquat could be detected at concentrations as low as 0.02 and 0.1 µg mL- 1 with the weak cation-exchange solid-phase extraction (WCX-SPE) method and dilution method for sample preparation, respectively. For quantitative analysis, the R2 cv of the partial least-squares regression model with the dilution method (0.939) was not as good as with the WCX-SPE method (0.984), but the dilution method is much less costly, simpler and time saving. Satisfactory recovery values were obtained ranging from 94.73% to 114.81%, with the exception of 56.55% for the lowest concentration. CONCLUSION: This work showed that SERS combined with gold nanoparticles could determine paraquat in apple juice. As a simple, rapid and ultrasensitive method, it has great practical potential for detection of other contaminants in a variety of foods.
Authors: Nguyen Ha Anh; Mai Quan Doan; Ngo Xuan Dinh; Tran Quang Huy; Doan Quang Tri; Le Thi Ngoc Loan; Bui Van Hao; Anh-Tuan Le Journal: RSC Adv Date: 2022-04-07 Impact factor: 3.361