| Literature DB >> 2936332 |
B Overdijk, E P Beem, G J van Steijn, L A Trippelvitz, J J Lisman, J Paz Parente, P Cardon, Y Leroy, B Fournet, H van Halbeek.
Abstract
The oligosaccharide structures of bovine brain beta-N-acetylhexosaminidases A and B (EC 3.2.1.30) were studied at the glycopeptide level by employing 500 MHz 1H-n.m.r. spectroscopy and methylation analysis involving g.l.c.-m.s. More than 90% of the chains were found to be of the oligomannoside type, containing, on average, five to six mannose residues. Biantennary N-acetyl-lactosamine-type chains terminated in N-acetylneuraminic acid were found to comprise the remaining 5-10% of the total carbohydrate. The isoenzyme forms A and B do not differ from each other in the structure of their carbohydrate moiety, but do deviate in carbohydrate content and, in consequence, in the number of carbohydrate chains per molecule.Entities:
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Year: 1985 PMID： 2936332 PMCID： PMC1152932 DOI： 10.1042/bj2320637
Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857