Literature DB >> 29356334

OMIP-044: 28-color immunophenotyping of the human dendritic cell compartment.

Florian Mair1, Martin Prlic1.   

Abstract

This work describes the first 30-parameter immunophenotyping of the human dendritic cell (DC) compartment using fluorescent-based flow cytometry. The optimized panel allows for simultaneous detection of 21 myeloid-centric markers distinguishing all canonical DC subsets, with parallel enumeration of monocytes, T and B cells as well as NK cells. Thus, this panel will be useful for extensive phenotyping of immune cells from a variety of human samples limited in size.
© 2018 International Society for Advancement of Cytometry.

Entities:  

Keywords:  antigen-presenting cells; dendritic cells; high-dimensional cytometry; human PBMCs; human tissue; immunophenotyping; myeloid cells

Mesh：

Substances：

Year:  2018        PMID： 29356334      PMCID： PMC6785241          DOI： 10.1002/cyto.a.23331

Source DB:  PubMed          Journal:  Cytometry A        ISSN： 1552-4922            Impact factor:   4.355


  30 in total

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  16 in total

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4.  OMIP-051 - 28-color flow cytometry panel to characterize B cells and myeloid cells.

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5.  Human MAIT cells exit peripheral tissues and recirculate via lymph in steady state conditions.

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