Bao-Cheng Zhao1, Ji-Dong Xu1, Bo-Yang Chen1, Xue-Fei Cao1, Tong-Qi Yuan2, Shuang-Fei Wang3, Adam Charlton4, Run-Cang Sun5. 1. Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, No. 35 Tsinghua East Road Haidian District, Beijing, 100083, China. 2. Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, No. 35 Tsinghua East Road Haidian District, Beijing, 100083, China. ytq581234@163.com. 3. College of Light Industry and Food Engineering, Guangxi University, No. 100 Daxue East Road, Nanning, 530000, China. 4. The BioComposites Centre, Bangor University, Deiniol Road, Bangor, Gwynedd, LL57 2UW, UK. 5. Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, No. 35 Tsinghua East Road Haidian District, Beijing, 100083, China. rcsun3@bjfu.edu.cn.
Abstract
MAIN CONCLUSION: Six types of lignin-carbohydrate complex (LCC) fractions were isolated from Eucalyptus. The acidic dioxane treatment applied significantly improved the yield of LCCs. The extraction conditions had a limited impact on the LCC structures and linkages. Characterization of the lignin-carbohydrate complex (LCC) structures and linkages promises to offer insight on plant cell wall chemistry. In this case, Eucalyptus LCCs were extracted by aqueous dioxane, and then precipitated sequentially by 70% ethanol, 100% ethanol, and acidic water (pH = 2). The composition and structure of the six LCC fractions obtained by selective precipitation were investigated by sugar analysis, molecular weight determination, and 2D HSQC NMR. It was found that the acidic (0.05-M HCl) dioxane treatment significantly improved the yield of LCCs (66.4% based on Klason lignin), which was higher than the neutral aqueous dioxane extraction, and the extraction condition showed limited impact on the LCC structures and linkages. In the fractionation process, the low-molecular-weight LCCs containing a high content of carbohydrates (60.3-63.2%) were first precipitated by 70% ethanol from the extractable solution. The phenyl glycoside (PhGlc) bonds (13.0-17.0 per 100Ar) and highly acetylated xylans were observed in the fractions recovered by the precipitation with 100% ethanol. On the other hand, such xylan-rich LCCs exhibited the highest frequency of β-O-4 linkages. The benzyl ether (BE) bonds were only detected in the fractions obtained by acidic water precipitation.
MAIN CONCLUSION: Six types of lignin-carbohydrate complex (LCC) fractions were isolated from Eucalyptus. The acidic dioxane treatment applied significantly improved the yield of LCCs. The extraction conditions had a limited impact on the LCC structures and linkages. Characterization of the lignin-carbohydrate complex (LCC) structures and linkages promises to offer insight on plant cell wall chemistry. In this case, Eucalyptus LCCs were extracted by aqueous dioxane, and then precipitated sequentially by 70% ethanol, 100% ethanol, and acidic water (pH = 2). The composition and structure of the six LCC fractions obtained by selective precipitation were investigated by sugar analysis, molecular weight determination, and 2D HSQC NMR. It was found that the acidic (0.05-M HCl) dioxane treatment significantly improved the yield of LCCs (66.4% based on Klason lignin), which was higher than the neutral aqueous dioxane extraction, and the extraction condition showed limited impact on the LCC structures and linkages. In the fractionation process, the low-molecular-weight LCCs containing a high content of carbohydrates (60.3-63.2%) were first precipitated by 70% ethanol from the extractable solution. The phenyl glycoside (PhGlc) bonds (13.0-17.0 per 100Ar) and highly acetylated xylans were observed in the fractions recovered by the precipitation with 100% ethanol. On the other hand, such xylan-rich LCCs exhibited the highest frequency of β-O-4 linkages. The benzyl ether (BE) bonds were only detected in the fractions obtained by acidic water precipitation.
Authors: Xueyu Du; Marta Pérez-Boada; Carmen Fernández; Jorge Rencoret; José C del Río; Jesús Jiménez-Barbero; Jiebing Li; Ana Gutiérrez; Angel T Martínez Journal: Planta Date: 2014-02-15 Impact factor: 4.116
Authors: Nghi H Do; Hieu H Pham; Tan M Le; Jeroen Lauwaert; Ludo Diels; An Verberckmoes; Nga H N Do; Viet T Tran; Phung K Le Journal: Sci Rep Date: 2020-12-04 Impact factor: 4.379
Authors: Zhi Chang Liu; Zi Wei Wang; Song Gao; Yu Xing Tong; Xi Le; Nian Wu Hu; Qun Shan Yan; Xian Gang Zhou; Yan Rong He; Lei Wang Journal: Front Bioeng Biotechnol Date: 2021-12-06