Bacterial nucleoid-associated proteins (NAPs) are critical to genome integrity and chromosome maintenance. Post-translational modifications of bacterial NAPs appear to function similarly to their better studied mammalian counterparts. The histone-like NAP HupB from Mycobacterium tuberculosis (Mtb) was previously observed to be acetylated by the acetyltransferase Eis, leading to genome reorganization. We report biochemical and structural aspects of acetylation of HupB by Eis. We also found that the SirT-family NAD+-dependent deacetylase Rv1151c from Mtb deacetylated HupB in vitro and characterized the deacetylation kinetics. We propose that activities of Eis and Rv1151c could regulate the acetylation status of HupB to remodel the mycobacterial chromosome in response to environmental changes.
Bacterial nun>an class="Chemical">cleoid-associated proteins (NAPs) are critical to genome integrity and chromosome maintenance. Post-translational modifications of bacterial NAPs appear to function similarly to their better studied mammaliancounterparts. The histone-like NAP HupB from Mycobacterium tuberculosis (Mtb) was previously observed to be acetylated by the acetyltransferase Eis, leading to genome reorganization. We report biochemical and structural aspects of acetylation of HupB by Eis. We also found that the SirT-family NAD+-dependent deacetylase Rv1151c from Mtb deacetylated HupB in vitro and characterized the deacetylation kinetics. We propose that activities of Eis and Rv1151ccould regulate the acetylation status of HupB to remodel the mycobacterial chromosome in response to environmental changes.
Authors: Atefeh Garzan; Melisa J Willby; Keith D Green; Chathurada S Gajadeera; Caixia Hou; Oleg V Tsodikov; James E Posey; Sylvie Garneau-Tsodikova Journal: J Med Chem Date: 2016-11-22 Impact factor: 7.446
Authors: Kyoung Hoon Kim; Doo Ri An; Jinsu Song; Ji Young Yoon; Hyoun Sook Kim; Hye Jin Yoon; Ha Na Im; Jieun Kim; Do Jin Kim; Sang Jae Lee; Ki-Hye Kim; Hye-Mi Lee; Hie-Joon Kim; Eun-Kyeong Jo; Jae Young Lee; Se Won Suh Journal: Proc Natl Acad Sci U S A Date: 2012-04-30 Impact factor: 11.205
Authors: Airlie J McCoy; Ralf W Grosse-Kunstleve; Paul D Adams; Martyn D Winn; Laurent C Storoni; Randy J Read Journal: J Appl Crystallogr Date: 2007-07-13 Impact factor: 3.304
Authors: Ankita Punetha; Keith D Green; Atefeh Garzan; Nishad Thamban Chandrika; Melisa J Willby; Allan H Pang; Caixia Hou; Selina Y L Holbrook; Kyle Krieger; James E Posey; Tanya Parish; Oleg V Tsodikov; Sylvie Garneau-Tsodikova Journal: RSC Med Chem Date: 2021-10-05
Authors: Monica Campo; Sarah Heater; Glenna J Peterson; Jason D Simmons; Shawn J Skerrett; Harriet Mayanja-Kizza; Catherine M Stein; W Henry Boom; Thomas R Hawn Journal: Tuberculosis (Edinb) Date: 2021-02-18 Impact factor: 3.131
Authors: Enzo M Scutigliani; Edwin R Scholl; Anita E Grootemaat; Sadhana Khanal; Jakub A Kochan; Przemek M Krawczyk; Eric A Reits; Atefeh Garzan; Huy X Ngo; Keith D Green; Sylvie Garneau-Tsodikova; Jan M Ruijter; Henk A van Veen; Nicole N van der Wel Journal: Front Microbiol Date: 2018-09-05 Impact factor: 5.640
Authors: D G Christensen; J T Baumgartner; X Xie; K M Jew; N Basisty; B Schilling; M L Kuhn; A J Wolfe Journal: mBio Date: 2019-04-09 Impact factor: 7.867