Goneta Gashi1, Vesna Mahovlić2, Suzana Manxhuka-Kerliu1, Arjeta Podrimaj-Bytyqi1, Luljeta Gashi3, Isa R Elezaj4. 1. a Department of Pathology, Faculty of Medicine , University of Prishtina , Prishtina , Kosovo. 2. b Department of Pathology and Cytology , Clinical Unit of Gynecological Cytology, University Hospital Center , Zagreb , Croatia. 3. c Department of Radiology, Faculty of Medicine , University of Prishtina , Prishtina , Kosovo. 4. d Department of Biology, Faculty of Natural Sciences , University of Prishtina , Prishtina , Kosovo.
Abstract
BACKGROUND AND AIM: The loss of genomic stability plays an important role in carcinogenesis. Therefore, it is imperative to use certain biomarkers of DNA damage due to genomic instability in order to predict cancer risk. The aim of this study was the evaluation of genomic instability in patients with cervical lesions. MATERIALS AND METHODS: We investigated the genetic damages in 80 subjects: 40 patients with high-grade squamous intraepithelial lesions (HSIL), 20 patients with invasive squamous cervical cancer (SCC) and 20 healthy women with a biomarker in two different tissues; the micronucleus (MN) test in peripheral blood lymphocytes (PBL), and in buccal exfoliated cells (BEC). This study also examined the frequency of other nuclear anomalies such as nucleoplasmic bridges (NPBs) and nuclear bunds (NBUDs) in PBL. RESULTS: The frequency of MN in BEC, MN in PBL, NPB in PBL and NBUD in PBL were significantly higher (p < 0.001), in patients compared to controls. The DNA damages in BEC and PBL were correlated positively with histological grade of cervical lesions. CONCLUSION: Although larger studies are needed, our data support the predictive value of MN, NPB and NBUD as biomarkers of genomic instability for evaluation of risk level of cancer diseases.
BACKGROUND AND AIM: The loss of genomic stability plays an important role in carcinogenesis. Therefore, it is imperative to use certain biomarkers of DNA damage due to genomic instability in order to predict cancer risk. The aim of this study was the evaluation of genomic instability in patients with cervical lesions. MATERIALS AND METHODS: We investigated the genetic damages in 80 subjects: 40 patients with high-grade squamous intraepithelial lesions (HSIL), 20 patients with invasive squamous cervical cancer (SCC) and 20 healthy women with a biomarker in two different tissues; the micronucleus (MN) test in peripheral blood lymphocytes (PBL), and in buccal exfoliated cells (BEC). This study also examined the frequency of other nuclear anomalies such as nucleoplasmic bridges (NPBs) and nuclear bunds (NBUDs) in PBL. RESULTS: The frequency of MN in BEC, MN in PBL, NPB in PBL and NBUD in PBL were significantly higher (p < 0.001), in patients compared to controls. The DNA damages in BEC and PBL were correlated positively with histological grade of cervical lesions. CONCLUSION: Although larger studies are needed, our data support the predictive value of MN, NPB and NBUD as biomarkers of genomic instability for evaluation of risk level of cancer diseases.
Entities:
Keywords:
Micronucleus; cancer risk; genome instability
Authors: Antonio Pedro Dos Reis Filho; Maruhen Amir Datsch Silveira; Natani Ribeiro Demarco; Luciana Paula Gregio D'Arce Journal: In Vivo Date: 2019 Nov-Dec Impact factor: 2.155