Literature DB >> 29334382

A de novo enzyme catalyzes a life-sustaining reaction in Escherichia coli.

Ann E Donnelly1, Grant S Murphy1, Katherine M Digianantonio1, Michael H Hecht1.   

Abstract

Producing novel enzymes that are catalytically active in vitro and biologically functional in vivo is a key goal of synthetic biology. Here we describe Syn-F4, the first de novo protein that meets both criteria. Purified Syn-F4 hydrolyzes the siderophore ferric enterobactin, and expression of Syn-F4 allows an inviable strain of Escherichia coli to grow in iron-limited medium. These findings demonstrate that entirely new sequences can provide life-sustaining enzymatic functions in living organisms.

Entities:  

Mesh：

Substances：

Year:  2018        PMID： 29334382     DOI： 10.1038/nchembio.2550

Source DB:  PubMed          Journal:  Nat Chem Biol        ISSN： 1552-4450            Impact factor:   15.040


  19 in total

1.  New Tricks for Old Proteins: Single Mutations in a Nonenzymatic Protein Give Rise to Various Enzymatic Activities.

Authors:  Yurii S Moroz; Tiffany T Dunston; Olga V Makhlynets; Olesia V Moroz; Yibing Wu; Jennifer H Yoon; Alissa B Olsen; Jaclyn M McLaughlin; Korrie L Mack; Pallavi M Gosavi; Nico A J van Nuland; Ivan V Korendovych
Journal:  J Am Chem Soc       Date:  2015-11-20       Impact factor: 15.419

Review 2.  De novo proteins from binary-patterned combinatorial libraries.

Authors:  Luke H Bradley; Peter P Thumfort; Michael H Hecht
Journal:  Methods Mol Biol       Date:  2006

3.  A designed supramolecular protein assembly with in vivo enzymatic activity.

Authors:  Woon Ju Song; F Akif Tezcan
Journal:  Science       Date:  2014-12-19       Impact factor: 47.728

4.  Rapid and efficient site-specific mutagenesis without phenotypic selection.

Authors:  T A Kunkel
Journal:  Proc Natl Acad Sci U S A       Date:  1985-01       Impact factor: 11.205

5.  Divergent evolution of a bifunctional de novo protein.

Authors:  Betsy A Smith; Ann E Mularz; Michael H Hecht
Journal:  Protein Sci       Date:  2014-12-26       Impact factor: 6.725

6.  In vitro characterization of salmochelin and enterobactin trilactone hydrolases IroD, IroE, and Fes.

Authors:  Hening Lin; Michael A Fischbach; David R Liu; Christopher T Walsh
Journal:  J Am Chem Soc       Date:  2005-08-10       Impact factor: 15.419

7.  Selection and evolution of enzymes from a partially randomized non-catalytic scaffold.

Authors:  Burckhard Seelig; Jack W Szostak
Journal:  Nature       Date:  2007-08-16       Impact factor: 49.962

8.  De novo designed proteins from a library of artificial sequences function in Escherichia coli and enable cell growth.

Authors:  Michael A Fisher; Kara L McKinley; Luke H Bradley; Sara R Viola; Michael H Hecht
Journal:  PLoS One       Date:  2011-01-04       Impact factor: 3.240

9.  Computational design of catalytic dyads and oxyanion holes for ester hydrolysis.

Authors:  Florian Richter; Rebecca Blomberg; Sagar D Khare; Gert Kiss; Alexandre P Kuzin; Adam J T Smith; Jasmine Gallaher; Zbigniew Pianowski; Roger C Helgeson; Alexej Grjasnow; Rong Xiao; Jayaraman Seetharaman; Min Su; Sergey Vorobiev; Scott Lew; Farhad Forouhar; Gregory J Kornhaber; John F Hunt; Gaetano T Montelione; Liang Tong; K N Houk; Donald Hilvert; David Baker
Journal:  J Am Chem Soc       Date:  2012-09-21       Impact factor: 15.419

10.  Functional proteins from a random-sequence library.

Authors:  A D Keefe; J W Szostak
Journal:  Nature       Date:  2001-04-05       Impact factor: 49.962
View more
  13 in total

1.  De novo design of symmetric ferredoxins that shuttle electrons in vivo.

Authors:  Andrew C Mutter; Alexei M Tyryshkin; Ian J Campbell; Saroj Poudel; George N Bennett; Jonathan J Silberg; Vikas Nanda; Paul G Falkowski
Journal:  Proc Natl Acad Sci U S A       Date:  2019-07-01       Impact factor: 11.205

2.  Protein evolution: Hacking an enzyme.

Authors:  Kristoffer E Johansson; Jakob R Winther
Journal:  Nat Chem Biol       Date:  2018-02-14       Impact factor: 15.040

3.  CoLiDe: Combinatorial Library Design tool for probing protein sequence space.

Authors:  Vyacheslav Tretyachenko; Václav Voráček; Radko Souček; Kosuke Fujishima; Klára Hlouchová
Journal:  Bioinformatics       Date:  2021-05-01       Impact factor: 6.937

4.  Minimalist de novo Design of Protein Catalysts.

Authors:  Liam R Marshall; Oleksii Zozulia; Zsofia Lengyel-Zhand; Ivan V Korendovych
Journal:  ACS Catal       Date:  2019-09-13       Impact factor: 13.084

Review 5.  De novo proteins from random sequences through in vitro evolution.

Authors:  Cher Ling Tong; Kun-Hwa Lee; Burckhard Seelig
Journal:  Curr Opin Struct Biol       Date:  2021-01-28       Impact factor: 7.786

Review 6.  Designed for life: biocompatible de novo designed proteins and components.

Authors:  Katie J Grayson; J L Ross Anderson
Journal:  J R Soc Interface       Date:  2018-08       Impact factor: 4.118

7.  De Novo Emergence of Peptides That Confer Antibiotic Resistance.

Authors:  Michael Knopp; Jonina S Gudmundsdottir; Tobias Nilsson; Finja König; Omar Warsi; Fredrika Rajer; Pia Ädelroth; Dan I Andersson
Journal:  mBio       Date:  2019-06-04       Impact factor: 7.867

Review 8.  Yeast-Based Biosensors: Current Applications and New Developments.

Authors:  Helene Martin-Yken
Journal:  Biosensors (Basel)       Date:  2020-05-13

Review 9.  The ascent of man(made oxidoreductases).

Authors:  Katie J Grayson; Jl Ross Anderson
Journal:  Curr Opin Struct Biol       Date:  2018-05-10       Impact factor: 6.809

10.  A Strategy for Combinatorial Cavity Design in De Novo Proteins.

Authors:  Christina Karas; Michael Hecht
Journal:  Life (Basel)       Date:  2020-01-23
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.