Literature DB >> 2932370

Nucleotide sequence of the GDH gene coding for the NADP-specific glutamate dehydrogenase of Saccharomyces cerevisiae.

T Nagasu, B D Hall.   

Abstract

The isolation of the Saccharomyces cerevisiae gene for NADP-dependent glutamate dehydrogenase (NADP-GDH) by cross hybridization to the Neurospora crassa am gene, known to encode for NADP-GDH is described. Two DNA fragments selected from a yeast genomic library in phage lambda gt11 were shown by restriction analysis to share 2.5 kb of common sequence. A yeast shuttle vector (CV13) carrying either to the cloned fragments complements the gdh- strain of S. cerevisiae and directs substantial overproduction of NADP-GDH. One of the cloned fragments was sequenced, and the deduced amino acid (aa) sequence of the yeast NADP-GDH is 64% homologous to N. crassa, 51% to Escherichia coli and 24% to bovine NADP-GDHs.

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Year:  1985        PMID： 2932370     DOI： 10.1016/0378-1119(85)90279-3

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  24 in total

1.  Uptake of ammonia by Saccharomyces cerevisiae carrying the plasmid pCYG4 related with ammonia assimilation.

Authors:  J L Lima Filho; W M Ledingham
Journal:  Appl Biochem Biotechnol       Date:  1992-08       Impact factor: 2.926

2.  The gene for a halophilic glutamate dehydrogenase: sequence, transcription analysis and phylogenetic implications.

Authors:  N Benachenhou; G Baldacci
Journal:  Mol Gen Genet       Date:  1991-12

3.  A simple program to calculate codon bias index.

Authors:  T T Wang; W C Cheng; B H Lee
Journal:  Mol Biotechnol       Date:  1998-10       Impact factor: 2.695

4.  Studies on Saccharomyces cerevisiae under carbon-limiting growth transformed with plasmid pCYG4 that carries the gene for NADP-GDH.

Authors:  J L Lima Filho; W M Ledingham
Journal:  Appl Biochem Biotechnol       Date:  1990-02       Impact factor: 2.926

5.  Nucleotide sequence and regulation of expression of the Aspergillus nidulans gdhA gene encoding NADP dependent glutamate dehydrogenase.

Authors:  A R Hawkins; S J Gurr; P Montague; J R Kinghorn
Journal:  Mol Gen Genet       Date:  1989-07

6.  Studies on Saccharomyces cerevisiae carrying the plasmid pCYG4 related with ammonia assimilation. Batch experiments.

Authors:  J L Lima Filho; W M Ledingham
Journal:  Appl Biochem Biotechnol       Date:  1988-10       Impact factor: 2.926

7.  The NADP+-linked glutamate dehydrogenase from Trypanosoma cruzi: sequence, genomic organization and expression.

Authors:  P Barderi; O Campetella; A C Frasch; J A Santomé; U Hellman; U Pettersson; J J Cazzulo
Journal:  Biochem J       Date:  1998-03-01       Impact factor: 3.857

8.  Regulatory circuit for responses of nitrogen catabolic gene expression to the GLN3 and DAL80 proteins and nitrogen catabolite repression in Saccharomyces cerevisiae.

Authors:  J R Daugherty; R Rai; H M el Berry; T G Cooper
Journal:  J Bacteriol       Date:  1993-01       Impact factor: 3.490

9.  Identification of potential target genes for Adr1p through characterization of essential nucleotides in UAS1.

Authors:  C Cheng; N Kacherovsky; K M Dombek; S Camier; S K Thukral; E Rhim; E T Young
Journal:  Mol Cell Biol       Date:  1994-06       Impact factor: 4.272

10.  Cloning, sequencing and expression of the Schwanniomyces occidentalis NADP-dependent glutamate dehydrogenase gene.

Authors:  P A De Zoysa; I F Connerton; D C Watson; J R Johnston
Journal:  Curr Genet       Date:  1991-08       Impact factor: 3.886
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