Literature DB >> 29321286

Oocyte Host-Transfer and Maternal mRNA Depletion Experiments in Xenopus.

Douglas W Houston1.   

Abstract

This protocol details the oocyte host-transfer method in Xenopus, using transplantation by intraperitoneal injection. This approach is suitable for the overexpression of mRNAs and for the use of antisense oligonucleotides to deplete maternal mRNAs, which are not replaced until zygotic genome activation in the mid-blastula transition. Xenopus oocyte host-transfer can also be used for highly efficient mutagenesis in the F0 generation by prefertilization injection of genome editing reagents.
© 2018 Cold Spring Harbor Laboratory Press.

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Year:  2018        PMID： 29321286      PMCID： PMC6538067          DOI： 10.1101/pdb.prot096982

Source DB:  PubMed          Journal:  Cold Spring Harb Protoc        ISSN： 1559-6095


  22 in total

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Authors:  R Brun
Journal:  Experientia       Date:  1975-11-15

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Journal:  Exp Cell Res       Date:  1962-06       Impact factor: 3.905

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Authors:  L D Smith; W L Xu; R L Varnold
Journal:  Methods Cell Biol       Date:  1991       Impact factor: 1.441

Review 5.  Regulation of cell polarity and RNA localization in vertebrate oocytes.

Authors:  Douglas W Houston
Journal:  Int Rev Cell Mol Biol       Date:  2013       Impact factor: 6.813

6.  Isolation of Xenopus Oocytes.

Authors:  Karen Newman; Tristan Aguero; Mary Lou King
Journal:  Cold Spring Harb Protoc       Date:  2018-02-01

7.  High-efficiency non-mosaic CRISPR-mediated knock-in and indel mutation in F0 Xenopus.

Authors:  Yetki Aslan; Emmanuel Tadjuidje; Aaron M Zorn; Sang-Wook Cha
Journal:  Development       Date:  2017-07-10       Impact factor: 6.868

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Authors:  Hazel L Sive; Robert M Grainger; Richard M Harland
Journal:  CSH Protoc       Date:  2007-05-01

Review 9.  The use of antisense oligonucleotides in Xenopus oocytes.

Authors:  Alissa M Hulstrand; Patricia N Schneider; Douglas W Houston
Journal:  Methods       Date:  2010-01-05       Impact factor: 3.608

10.  Manipulation and in vitro maturation of Xenopus laevis oocytes, followed by intracytoplasmic sperm injection, to study embryonic development.

Authors:  Kei Miyamoto; David Simpson; John B Gurdon
Journal:  J Vis Exp       Date:  2015-02-09       Impact factor: 1.355
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  3 in total

1.  Using the Xenopus Oocyte Toolbox.

Authors:  Kimberly L Mowry
Journal:  Cold Spring Harb Protoc       Date:  2020-04-01

2.  Novel functions of the ubiquitin-independent proteasome system in regulating Xenopus germline development.

Authors:  Hyojeong Hwang; Zhigang Jin; Vishnu Vardhan Krishnamurthy; Anumita Saha; Peter S Klein; Benjamin Garcia; Wenyan Mei; Mary Lou King; Kai Zhang; Jing Yang
Journal:  Development       Date:  2019-04-23       Impact factor: 6.868

3.  Trim-Away mediated knock down uncovers a new function for Lbh during gastrulation of Xenopus laevis.

Authors:  Emma Weir; Gretchen McLinden; Dominique Alfandari; Hélène Cousin
Journal:  Dev Biol       Date:  2020-11-04       Impact factor: 3.582
  3 in total

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