Prevalence of Toxoplasma gondii Infection among Healthy Blood Donors in Northeast of Iran.

BACKGROUND: This cross-sectional investigation aimed to evaluate the prevalence of IgM and IgG anti-Toxoplasma gondii antibodies and the associated risk factors among healthy blood donors in Khorasan Razavi Province, northeast of Iran from Nov 2014 to May 2015.
METHODS: Overall, 491 serum samples from apparently healthy blood donors referred the six biggest blood centers in Razavi Khorasan, Iran, were screened for IgG and IgM anti-T. gondii antibodies by enzyme-linked immunosorbent assay (ELISA). A structured questionnaire was used to obtain information on risk factors for T. gondii infection. Nested PCR was also used to detect DNA of T. gondii in the IgM-positive samples by using of B1 and RE (Repetitive Element) as marker for amplifying fragment size of 531 bp and 164 bp in PCR method.
RESULTS: Totally, 200 (40.7%) samples were seropositive for anti-T. gondii antibodies; 184 (37.5%) donors tested seropositive for only IgG antibody, 8 (1.6%) tested seropositive for both IgM and IgG and 8 (1.6%) were positive for IgM antibody alone. Several risk factors significantly related to T. gondii seropositivity in the univariate analysis at P<0.05 included age (P<0.001), and raw/half-cocked meat consumption (P=0.015). T. gondii DNA was found in all sixteen IgM-positive samples.
CONCLUSION: T. gondii infection was present among healthy blood donors in northeast of Iran. Thus, it is suggested to design screening programs for preventing transfusion-transmitted toxoplasmosis.

Introduction

Toxoplasma gondii, an obligate intra-cellular parasite, is a generally successful microorganism that infects around 30% of the human population globally (1). The transmission of infection occurs by ingestion of water, vegetables and/or soil contaminated with oocysts from cat feces; or raw/undercooked meat containing tissue cysts, and congenitally (2, 3). Toxoplasma infection may be transmitted via the whole blood or white blood cell transfusions or organ transplantation to vulnerable recipients (3, 4).

Among immunocompetent people, toxoplasmosis is generally asymptomatic, whereas, the infection in the immunocompromised individuals such as patients with acquired immunodeficiency syndrome, and neonates can be fatal for their living (5–8). Considering a number of factors for example community and cultural behavior, climate, and form of transmission, the prevalence of toxoplasmosis is varying from 10% to 80% (9–12). In Iran, the prevalence of this infection varies depending on geographical regions was approximately 18% to 70% (13). At present time, there are numerous serological investigations on the prevalence of T. gondii antibodies between blood donors in various regions of the world (14–24); however, there are a small number of studies on seroprevalence of toxoplasmosis in healthy blood donors of Iran (25–30).

The present cross-sectional investigation aimed to determine the prevalence of IgM and IgG anti-T. gondii antibodies and the associated risk factors among healthy blood donors in Razavi Khorasan Province, northeastern Iran. Moreover, as a second objective, to confirm the presence of T. gondii DNA and parasitemia in blood donors, all IgM-positive analyzed using molecular tests with diagnostic markers.

Materials and Methods

Study design

This cross-sectional study was carried out in the six biggest blood centers of Razavi Khorasan Blood Transfusion Organization (RKBTO) in Razavi Khorasan, Iran. This province covers an area of 144681 km2 with the population of nearly 6000000 and is located in the northeast of Iran.

Sample collection and participants

A total of, 491 serum samples were collected from apparently healthy blood donors referred the six biggest blood centers of RKBTO in Razavi Khorasan, Iran, during the period from Nov 2014 to May 2015.

Questionnaire

The applied questionnaire anchored in demographic data including age, gender, education, residence, and blood group was prepared before collection of blood samples. Moreover, possible risk factors, such as animal contacts (cats), raw/half-cooked meat consumption (lamb and beef), consumption of raw vegetables and raw egg/milk, gardening or agriculture activity, and blood transfusion were evaluated.

ELISA test

To evaluate the anti-T. gondii antibodies, all the serum samples were tested using the commercially available ELISA kit (Dia.Pro, Italy). Analyses were performed following the manufacturer’s instructions. Based on the ELISA kit, positive results for IgG and IgM were defined as values of ≥500 international units (IU)/ml and index values of ≥0.6, respectively. Range of equivocal results was from 250 to 500 IU/ml and index values of 0.5 to 0.6 were assumed for IgG and IgM, respectively. In addition, negative results were defined as < 250 IU/ml and index values of < 0.5 were considered for IgG and IgM, respectively.

Molecular study using Nested PCR

Nested PCR was used to detect DNA of T. gondii in the IgM-positive samples. DNA was extracted from the buffy coat of all of the IgM positive samples according to the method described elsewhere (27). DNA of each sample was extracted using the DNeasy blood and tissue kit (Bioneer, South Korea) according to manufacturer’s instructions. DNA was stored at −20 °C until further use in PCR analysis. Specific primers related to both regions of B1 and RE (Repetitive Element) were used. PCR primers used for B1 gene amplification are as follows: Pml/S1, 5-TGTTCTGTCCTATCGCAACG (positions 128 to 147); Pml/S2, 5-TCTTCCCAGACGTGGATTTC (positions 152 to 171); Pml/AS1, 5-ACGGATGCAGTTCCTTTCTG (positions 707 to 688); and Pml/AS2, 5-CTCGACAATACGCTGCTTGA (positions 682 to 663). PCR primers used for RE gene amplification are as follows: RE nested PCR1, 5′-TGACTCGGGCCCAGCTGCGT (positions 71 to 90); RE nested PCR1, 5′-CTCCTCCCTTCGTCCAAGCCTCC (positions 490 to 468); RE nested PCR2, 5′-AGGGACAGAAGTCGAAGGGG (positions 187 to 206); and RE nested PCR2, 5′-GCAGCCAAGCCGGAAACATC (positions 350 to 331) (31–33).

Each reaction was carried out in a final volume of 25 μl, containing 1μl of each primer, 10-μl water, 8-μl of master (Ampliqon™) and 5μl of DNA for both B1 and RE gene.

The target B1 gene amplified using the following conditions: one cycle of 3 min initial denaturation at 94 °C followed by 35 cycles of 94 °C for 30 sec, 60 °C for 1 min,72 °C for 2 min and was ended by one cycle of final extension at 30 °C for 1 min. Reaction for RE were started with an initial denaturation at 94 C for 3 min, and then cycled 30 times with denaturation at 94 °C for 30 sec, follow by annealing at 60 °C for 30 sec, and finally an extension step at 72 °C for 2 min follow by 1 min final extension at 30 sec. PCR products of second round of the PCR were loaded onto a 1.5% agarose gel and the results were compared with standard band markers of T. gondii, 531 bp.

Ethics

Ethics Committee of Kerman University of Medical Sciences approved this study. In addition, a written informed consent was obtained from all the participants before blood sampling.

Statistical analyses

Statistical analysis was carried out using SPSS 17.0 software (Inc., Chicago, IL, USA). Logistic regression models were used to evaluate univariate between T. gondii seropositivity and the potential risk factors. Multivariate logistic analysis was performed with the full model, including all potential risk factors in the analyses. In this survey, P<0.05 was considered to be statistically significant.

Results

Participants

Overall, 491 blood donors were included in the present study; the mean age of the participants was 36.29±4.16 yr old (ranging from 18 to 57 yr old). Most participants were male (93.9%), aged 30–40 yr old, living in urban areas, which had college education or above.

Seroprevalence of anti-T. gondii antibodies

From 491 blood donors, 200 (40.7%) samples were seropositive for anti-T. gondii antibodies; 184 (37.5%) donors tested seropositive for only IgG antibody, 8 (1.6%) tested sero-positive for both IgM and IgG and 8 (1.6%) were positive for IgM antibody alone; indicating the seroprevalence of IgG and IgM anti-T. gondii antibodies were 37.5% and 3.2%, respectively. Regarding geographical region, seroprevalence of anti-T. gondii IgG antibody in six blood centers including Mashhad, Quchan, Torbat-e Heydarieh, Nishapur, Sabzevar, and Gonabad were 33.5, 35, 50, 41.6, 37.7, and 35% respectively. Seroprevalence of anti-T. gondii IgG antibodies were significantly higher among female donors; whereas, no significant difference was observed in the prevalence of IgM anti-T. gondii among the female and male donors (P=0.811). Statistical analysis also demonstrated that a significant difference in the prevalence of IgG (P≤0.001) and IgM (P=0.95) anti-T. gondii antibodies among different age groups (Table 1).

Table 1:

Demographic characteristics and T. gondii sero-prevalence among healthy blood donors in Razavi Khorasan Province, Iran

Variables	No. (%)	IgG positive	IgM positive
Gender
Male	461 (93.9)	(37.3)	(2.4)
Female	30 (6.1)	(40)	16.7
Age group(yr)
18–30	161 (32.8)	36	0
30–40	170 (34.6)	39.4	0.58
40–50	116 (23.6)	34.5	4.3
50–60	42(8.6)	42.8	23.8
>60yrs	2 (4)	0.5	0
Residential place
Urban	421 (85.5)	38.2	2.9
Rural	70 (14.3)	32.9	5.7
Education
Less than diploma	200 (40.7)	29.5	4.5
Diploma and above	291 (59.3)	43	2.4
Blood type
A	150 (30.5)	35.3	4
B	150 (30.5)	33.3	3.3
AB	46 (9.4)	39.1	2.2
O	145 (29.5)	43.4	2.8
Being in contact with cat
No	478 (97.4)	37.7	3.1
Yes	13 (2.6)	308	7.7
Raw/half-cooked meat consumption
No	443 (90.2)	37.7	2.9
Yes	48 (9.8)	35.4	6.3
Gardening or agriculture
No	346 (70.5)	38.2	3.5
Yes	145 (29.5)	35.9	2.8
Blood transfusion
No	478 (97.4)	37.4	3.3
Yes	13 (2.6)	38.5	0

Risk factors for being anti-T gondii antibodies

Several risk factors significantly related to T. gondii seropositivity in the univariate analysis at P<0.05 included age (P<0.001), and raw/half-cocked meat consumption (P<0.01). However, other demographic and risk factors of the blood donors did not show any correlation with T. gondii seropositivity (Table 2). The correlation between risk factors and status of anti-T. gondii IgG antibodies in the univariate analysis (crude OR) are shown in Table 2.

Table 2:

Univariate (crude OR) logistic regression analysis of the potential factors associated with T. gondii IgG seroprevalence among healthy blood donors in Razavi Khorasan Province, Iran

Variables	IgG Positive
	Crude OR (95% CI)	P value
Gender
Male	1	-
Female	1.5 (0.7, 3.1)	0.29
Age groups (yr)
18–30	-	-
30–40	-	-
40–50	-	-
50–60	-	-
>60	1.6	0.001*
Residential place
Urban	1	0.64
Rural	1.13 (0.675, 1.9)
Education
Less than diploma	1	-
Diploma and above	1.5 (1.15, 2.3)	0.14
Blood type
A	1	-
B	0.97 (0.16, 1.5)	0.91
AB	0.59 (0.29, 1.21)	0.15
O	0.84 (0.52, 1.34)	0.46
Being in contact with cat
No	1	-
Yes	1.44 (0.47, 4.36)	0.51
Raw/half-cooked meat consump-
tion	1	-
No	2.1 (1.14, 3.88)	0.018*
Yes
Gardening or agriculture
No	1	-
Yes	1.16 (0.78, 1.73)	0.45
Eating uncooked vegetables No
No	1	-
Yes	1.27 (0.65, 2.46)	0.49
Blood transfusion
No	1	-
Yes	0.49 (0.13,1.81)	0.29

In multiple logistic regression, age (P<0.001), and raw/half-cocked meat consumption were independent risk factors for Toxoplasma seropositivity.

Nested-PCR

To confirm the presence of T. gondii DNA in IgM-positive blood donors, all IgM-positive were analyzed using B1 and RE primers (Fig. 1 and 2). The findings revealed that T. gondii DNA was found in all sixteen IgM-positive samples. For all of samples, fragment of about 531, 164 bp were successfully PCR-amplified within B1 and RE genes, respectively. Therefore, there was a statistically significant association between IgM positivity and PCR results (P<0.05).

Fig. 1:

B1-nested PCR analysis of T. gondii DNA (531 bp). Lane 1–16:IgM-positive blood samples; C-: negative control (D.W); C+: positive control (T.gondii RH strain); ntc: negative test control (T.gondii negative sample); (ladder (100 bp)

Fig. 2:

RE-nested PCR analysis of T. gondii DNA (164bp). Lane 1–16:IgM-positive blood samples; nc: negative control (D.W); pc: positive control (T.gondii RH strain); ladder (100 bp)

Discussion

Toxoplasmosis in immunocompromised persons could cause severe manifestations such as central nervous system involvements as encephalitis and some brain disorders. This infection in blood donors potentially indicates a threat of parasite transmission to immunocompromised or immunosuppressed blood transfusion recipients (5–8). Because of in-progress treatments are not efficient and there is no successful vaccine, it is obligatory to perform labors for preventing of Toxoplasma transmission to blood recipients for likely incidence of reactivated toxoplasmosis specifically in immunodeficient persons. In this investigation, totally 491 blood samples were collected from blood donors of Razavi Khorasan, Iran. Overall, 200 (40.7%) samples were seropositive for anti-T. gondii antibodies; so that 37.5% and 3.2% were positive for IgG and IgM anti-T.gondii antibodies, respectively.

Many studies have reported similar T. gondii seropositivity in the blood donors of Czech Republic (19), Mexico (22), and southeastern Iran (25, 29, 30); whereas this T. gondii seropositivity was more than those reported in India (14), northeast of Thailand (23), and Taiwan (17), and some regions of Iran (26, 28, 30). In contrast, T. gondii seropositivity in the blood donors of Razavi Khorasan, Iran was less than the one reported among blood donors in central Iran (26), northeast of Brazil (30), north of India (16), and Egypt (15), where the seroprevalence of T. gondii has varied from 50% to 75%. This difference in T. gondii seropositivity among the blood donors around the world could be associated to some factors such as geographical and environmental factors, sociocultural habits, transmission routes, sample size in the studied population (9–12). Here, consumption of raw/half-cocked meat is the main risk factor for T. gondii seropositivity, indicating that among the blood donors in this study, the consumption of tissue cysts in meat (food-borne transmission) is one of the main routs of infection. Similarly, several studies have reported the food-borne transmission as main infection route in blood donors (26, 34, 35).

By gender, although seroprevalence of anti-T. gondii antibodies in female donors were higher male donor, but there is no significant difference in seroprevalence of anti-T. gondii antibodies among female and male donors. Recently, seroprevalence of T. gondii in female donors was significantly higher than that in males (18, 25). In contrast, T. gondii seropositivity was significantly higher among male donors (14, 26). Increasing of anti-T. gondii antibodies among female donors might be related to more exposure of females to oocysts and tissue cysts during their daily activities. However, because of a small number of female donors in this investigation, the present findings should be confirmed on a larger sample population.

Similar to the other studies we found that T. gondii seropositivity increased with age, indicated age because of increased opportunity for exposure; such a finding was in agreement with those observed in other studies (15, 24, 36).

There was no significant association between education, residence, blood group, raw-milk/egg consumption, and blood transfusion, agriculture activity, and contact with cat and seroprevalence of anti-T. gondii antibodies in blood donors.

By Nested-PCR analysis, T. gondii DNA was found in all sixteen IgM-positive samples. The presence of parasitemia in IgM-positive healthy blood donors, whereas ensures the likelihood of transmission of Toxoplasma through blood transfusion. Parasitemia in two (1.9%) of the IgM-positive subjects were demonstrated from blood donors in southern Iran (27). Moreover, T. gondii DNA was reported in one (9.0%) of IgM-positive samples healthy blood donors from Kerman Province, southeast of Iran (25). These variations could be related to some factors such as the short duration of parasitemia and the low numbers of trophozoites circulating in peripheral blood, which caused false negative results in such cases (37).

Conclusion

T. gondii infection was prevalent among healthy blood donors of Razavi Khorasan Province, in the northeastern of Iran with the overall seroprevalence rate of 40.7% and presence of parasite DNA of T. gondii in IgM-positive samples. Ingestion of undercooked meat is associated with increase of seropositivity in the blood donors in the northeast of Iran. The results can be a warning for blood transfusion organizations in order to pay special attention to toxoplasmosis among blood donors and design screening programs as preventive affairs for any probable transmission of toxoplasmosis.